黄芪总提物及其有效成分改善阿霉素致心衰的研究

目的:探讨黄芪总提物及其有效成分(黄芪甲苷、黄芪多糖、黄芪总皂苷)对阿霉素致心衰(HF)大鼠心脏血流动力学的改善作用.方法:90只雄性SD大鼠随机分为对照(Control)组、HF组、地高辛(DG)组、黄芪甲苷(ASIV)组、黄芪多糖( APS)组、黄芪总皂苷(AST)组和黄芪混合提取物(AEM)组,除HF组18只,其余各组均12只.采用阿霉素隔日腹腔注射致慢性HF模型,治疗药物DG,ASIV,APS,AST剂量分别为0.05,50,1 000,1 00 mg? kg-1,AEM相当于生药8 g?kg-1,每天灌胃给药.实验采用Langendorff联合多道生理记录仪进行心功能指标观察,记录分析比较各组的动物存活率(SR),左心室收缩压(LVSP)、心率(HR)及左心室收缩最大上升速率(+dp/dtmax)和左心室开始收缩至射血的间隔时间(T-dp/dtmax)的变化.结果:阿霉素所致HF模型与对照组进行比较各指标均明显下降(P＜0.01)；与HF组比较,AEM及其有效成分各组阿霉素致HF大鼠的SR,LVSP和+dp/dtmax均见明显提高,其中以AEM组在心脏收缩力学指标上的改善作用更为明显,APS组亦能显著提高LVSP及+dp/dtmax,能够增加心肌收缩力和改善左心室收缩最大上升速率,对HF具有一定的治疗作用.结论:AEM及其有效成分均可以提高阿霉素致HF大鼠的存活率,明显增加实验性HF大鼠的心肌收缩力,APS也显示了明显的疗效.

Effects of Astragalus Extract Mixture and its Effective Components on the Cardiac Function in the Rats with Experimental Heart Failure Induced by Adriamycin

Objective: To investigate the effects of astragalus extract mixture and effective components on the cardiac functions in rat heart failure(HF) induced by adriamycin. Method: Ninety male SD rats were randomized into 7 groups, including control group, HF group, Digoxigein (DG) group, Astragaloside (ASIV) group, astragalus polysaccharide (APS) group, total saponins of astragalus (AST) group and Astragalus Extract Mixture (AEM) group. Twelve rats were used in each of all groups except HF group in which 18 rats were used. The animals in all experimental groups except control group were intra-peritoneally injected with adriamycin(ADM) every other day to produce HF model; the animals in all experimental groups except HF group were respectively treated with intragastric administration of DG, AEM or other effective components aboved at the same time when ADM was given. The drugs dose of DG,ASIV,APS,AST were 0.05, 50,1 000, 100 and 8 000 mg·kg^-1respectively, The dose of Astragalus Extract Mixture in the AEM group is equivalent to the crude herb, Volume of gastric perfusion was 2 mL in the every groups. Survival Rate(SR), left ventricular systolic pressure(LVSP), Heart Rate(HR), +dp/dt_max and T-dp/dt_max were recorded and compared among the all groups. Result: The values of SR, LVSP, HR and +dp/dt_max in HF group were significantly lower than those in all other groups (P<0.01). As compared with HF group, SR, LVSP and +dp/dt_max were significantly enhanced in all experimental groups among which, the indices of cardiac function in AEM group were improved more significantly. Further experiment showed that AEM can also increase HR and shorten T-dp/dt_max in HF rats. Comparatively, the therapeutic effect of AEM in HF rats was even stronger than DG as shown in the present study. The parameters such as LVSP and +dp/dt_max in APS group were improved, so that the therapeutic action of the APS to HF can be demonstrated. Conclusion: Astragalus extract mixture and the effective components of astragalus including ASIV, AST and APS can significantly enhance the survival rate and cardiac contractility in the rats with experimental heart failure. Astragalus extract mixture may be more effective and safer in the treatment of HF. Curative effect of APS to HF is so benificial and significant that can not ignored.