MMP-2及PTEN在人脑星形细胞瘤中的表达及意义

目的探讨基质金属蛋白酶2(MMP-2)及抑癌基因PTEN在人脑星形瘤中的表达及二者与人脑星形细胞瘤侵袭性的关系。方法用免疫组织化学SABC法检测50例人脑星形细胞瘤组织和10例正常人脑组织中的MMP-2和PTEN蛋白的表达,并且分析二者与人脑星形细胞瘤临床病理分级的关系。结果 MMP-2和PTEN在低度恶性星形细胞瘤和高度恶性星形细胞瘤组织中表达差别有统计学意义(p<0.05)。随着星形细胞瘤恶性度增高,MMP-2的表达强度呈上升趋势而PTEN表达强度逐渐下降;Spearman等级相关分析表明人脑星形细胞瘤中MMP-2和PTEN之间呈负相关(Rs=-0.518,P<0.01)。结论 MMP-2和PTEN是人脑星形细胞瘤分化程度和转移的潜在生物学指标,联合检测MMP-2和PTEN更有利于判断星形细胞瘤生物学行为和病理分级。

Expression and significance of matrix metalloproteinase-2 and PTEN in human brain astrocytoma

Objective To investigate the expressions of MMP-2 and the tumor suppressor genes PTEN in human brain astrocytoma and their relationships between the expressions and tumor invasion.Methods The expressions of MMP-2 and phosphatase and tensin homolog deleted on chromosome ten(PTEN) protein were examined by immunohistochemistry(SABC method) in 50 human brain astrocytoma tissues and 10 nomal brain tissues,and their relationships of clinicopathological factors of human brain astrocytoma were analyzed.Results The expression rates of MMP-2 and PTEN had significantly difference between low grade human brain astrocytoma tissues and high human brain astrocytoma tissues.In nomal brain tissues(P<0.01),as the tumor’s malignancy degree increased,the expression of MMP-2 increased but the expression of PTEN decreased.The expression of MMP-2 was negatively correlation with the expression of PTEN in hunman brain astrocytoma(Rs=-0.518,P<0.01).Conclusions MMP-2 and PTEN are potential markers for differentiation and metastasis of human brain astrocytoma.Combined detection of MMP-2 and PTEN can estimate the biological behaviour and pathological grade.