中国北方肝细胞癌与乙肝病毒感染的紧密关联性

目的确立乙肝病毒(hepatitis B virus，HBV)及丙肝病毒(hepatitis C virus，HCV)感染在中国北方肝细胞癌(hepatocellular carcinoma，HCC)发生中的病因学地位。方法采用Abbott AxSym系统检测119例连续系列中国北方地区HCC病例的血清HBV、HCV标志，对血清HBV标志阴性病例，采用免疫组化、巢式PCR和DNA测序等对HBVX基因进行鉴定。结果119例HCC中，血清HBsAg阳性率为82．4％(98／119)；anti-HBc阳性率为94．1％(112／119)；4例血清HBsAg及anti-HBc均阴性病例中，3例HBVX基因阳性，因而HBV感染的阳性率达99．2％(118／119)。本组anti-HCV阳性率11．8％(14／119)，且均伴有HBV感染标志。结论中国北方地区HCC与HBV感染具有紧密关联性，是本地区HCC发生的主导病因。HCV感染是一个HOC发生的重要辅助因素。

Close relation of hepatocellular carcinoma to HBV infection in North China

Objective To define the importance of HBV and HCV infection in pathogenesis of hepatocellular carcinoma (HCC) in North China. Methods A consecutive series of 119 patients with HCC living in North China who were treated in our hospital were included in this study. Serum HBsAg, anti-HBc and anti-HCV were detected by Abbotts AxSym System. For those patients with negative detection of HBV sero-markers, HBV X gene was analyzed with nested PCR, DNA sequencing and immunohistochemistry for its expression. Results Amongst the 119 patients, 98 (82.4%) were HBsAg sero-positive. When a comprehensive set of HBV markers were detected, the rate of HBV infection in these patients reached the level of 99.2% (118/119). Of the 119 cases, 14 (11.8%) were found to be anti-HCV positive. However, all the anti-HCV positive cases had co-infection of HBV. Conclusions In North China, HBV infection is closely related to pathogenesis of HCC. Meanwhile, HBV infection is the major cause for HCC in this region. HCV is an important auxiliary factor for HCC.