大豆胚轴对糖尿病大鼠脂蛋白氧化拮抗作用

目的研究大豆胚轴提取物对糖尿病大鼠血浆脂蛋白氧化的抑制作用。方法从大豆胚轴提取总糖甙,高效液相色谱法(HPLC)分析其有效成分。给糖尿病大鼠饲喂添加100g/kg大豆胚轴提取物的饲料20周,观察血浆脂蛋白过氧化脂质(LPO)含量及氧化易感性的变化。结果饲喂大豆胚轴提取物后实验组糖尿病大鼠血浆LPO、低密度脂蛋白(LDL)-LPO和高密度脂蛋白(HDL)-LPO分别为(17.1±2.2),(2.6±0.5),(3.9±0.7)μmol/L,较对照组大鼠(21.4±3.5)、(3.4±0.8)和(5.4±0.9)μmol/L明显减少;在体外进行氧化修饰时,实验组大鼠LDL氧化延滞时间为(120±36)min,较对照组大鼠(83±27)min明显延长。结论大豆胚轴提取物可降低脂蛋白过氧化程度和增强LDL抗氧化修饰能力,有防治糖尿病及其并发症的作用。

Inhibition of soybean hypocotyl on oxidation of lipoprotein in diabetic rats

ObjectiveTo study the inhibitory effects of soybean hypocotyl extracts on oxidation of plasma lipoproteins in diabetic rats.MethodsTotal glycosides were extracted from soy bean hypocotyl,then detected with high-performance liquid chromatography(HPLC).Diabetic rats were given feed containing 100 g/kg soybean hypocotyl extracts for 20 weeks,and lipid peroxide(LPO)levels of lipoproteins and susceptibilities of lipoproteins to oxidation were investigated.ResultsLPO levels of plasma,LDL and HDL fractions of the experimental rats were(17.1±2.2),(2.6±0.5)and(3.9±0.7)mol/L, respectively after soy hypocotyl extract intake,which were significantly decreased than those of control rats[(21.4±3.5),(3.4±0.8)and(5.4±0.9)mol/L];lag phage of LDL oxidation curve in vitro was(120±36)min,noticeably prolonged than that of of control rats[(83±27)min].ConclusionIt is suggested that soy hypocotyl extracts,which can decrease the peroxidizability of lipoproteins and promote resistance to LDL oxidation,may be useful in prevention and treatment of diabetes mellitus and diabetes-associated diseases.