TGF-β1基因Leu10Pro多态性与T2DM肾病的相关性研究 |
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引用本文: | 魏明,卢永中,李素娟,佘红纯.TGF-β1基因Leu10Pro多态性与T2DM肾病的相关性研究[J].中华糖尿病杂志,2008,16(9):538-540. |
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作者姓名: | 魏明 卢永中 李素娟 佘红纯 |
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作者单位: | [1]郑州大学第五附属医院中心实验室,450052 [2]郑州铁路职业技术学院临床医学系,450052 |
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摘 要: | 目的探讨转化生长因子β1(TGF-β1)基因Leu10Pro多态性在郑州市汉族健康人群和糖尿病肾病(DN)患者中的分布以及与DN的相关性,并比较不同基因型个体血清TGF-β1表达水平。方法采用PCR-SSP法检测DN组患者280例、非DN(Non-DN)组患者280例和正常对照(NC)组270名TGF-β1基因Leu10Pro多态性,ELISA法检测血清TGF-β1水平。结果DN组TGF-β1基因Leu10Pro基因型频率和等位基因频率分布与Non-DN组和NC组比较差异有统计学意义(P均〈0.05)。Leu/Pro基因型携带者患DN的风险是Leu/Leu纯合子基因型的1.615倍;含等位基因Pro的基因型(Pro+)携带者、DN患者血清TGF-β1水平显著高于非携带者、DN组和NC组(P均〈0.05)。结论TGF-β1基因Leu10Pro多态性与DN的发病有关联,并影响DN患者血清TGF-β1表达水平,提示Pro等位基因可能是DN发病的一个危险因子。
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关 键 词: | 转化生长因子 糖尿病 2型 肾病 等位基因 基因型 |
Study of relationship between TGF-β Leu 10 Pro polymorphism and diabetic nephropathy in type 2 diabetes |
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Institution: | WEI Ming , LU Yong-shen , LI Su-juan ,et al.(Department of Clinical Laboratory, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.) |
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Abstract: | Objective To explore the relationship between TGF-β1 Leu10 Pro polymorphism and diabetic nephropathy in Chinese Han people. Methods The genotype of TGF-β1 was detected by PCR-SSP method in 280 patients with diabetic nephropathy (DN), 280 patients without diabetic nephropathy (Non-DN) and 270 healthy controls(NC), and the serum level of TGF-β1 was measured by enzyme-linked immunosorbent assay (ELISA). Results There was a significant difference in frequencies of allele and genotype in Leu10 Pro polymorpbism between DN and Non-DN or NC (all P〈0. 05). The relative risk of Leu/Pro genotype was 1. 615 times the Leu/Leu genotype (OR 1. 615, 95% CI: 1. 012-2. 437). The serum TGF-131 level was higher in genotypes with allele Pro than in Leu/Leu genotype(41.95 ± 8. 94)μg/L vs(35. 13 ± 5.33)μg/L, P〈0. 05]. DN group showed significantly higher serum level of TGF-βI versus Non-DN and control group(all P〈0. 05), Conclusion TGF-β1 polymorphism is associated with DN, Pro allele may be a risk factor for DN, its polymorphism may affect the serum level of TGF-β1. |
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Keywords: | Transforming growth factor Diabetes mellitus type 2 Nephropathy Allele Genotype |
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