| HPLC法测定杠板归中绿原酸的含量 |
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| 引用本文： | 茅向军,鲍家科,许乾丽,左鼎,王德甫. HPLC法测定杠板归中绿原酸的含量[J]. 贵阳医学院学报, 2010, 35(6): 567-569 |
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| 作者姓名： | 茅向军 鲍家科 许乾丽 左鼎 王德甫 |
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| 作者单位： | 贵州省药品检验所; |
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| 基金项目： | 贵州省中药现代化科技产业研究开发专项项目 |
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| 摘 要： | 目的：建立用高效液相色谱法测定杠板归中绿原酸的含量的方法。方法：采用高效液相色谱法,Hypersil ODS2 C18柱,流动相为乙腈-0.4%磷酸（10∶90）,检测波长为327 nm。结果：绿原酸在0.042～1.14μg范围内线性关系良好,平均回收率为99.19%,RSD为1.7%。结论：本分析方法简便、准确,可作为该药材质量控制的定量分析方法。
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| 关 键 词： | 杠板归 绿原酸 色谱法,高压液相 |
Determination of Chlorogenic Acid in Polygonum perfoliatum L.with High Performance Liquid Chromatography |
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| MAO Xiangjun,BAO Jiake,XU Qianli,ZUO Ding,WANG Defu. Determination of Chlorogenic Acid in Polygonum perfoliatum L.with High Performance Liquid Chromatography[J]. Journal of Guiyang Medical College, 2010, 35(6): 567-569 |
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| Authors: | MAO Xiangjun BAO Jiake XU Qianli ZUO Ding WANG Defu |
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| Affiliation: | MAO Xiangjun,BAO Jiake,XU Qianli,ZUO Ding,WANG Defu(Guizhou Provincial Institute for Drug Control,Guiyang 550004,Guizhou,China) |
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| Abstract: | Objective： To establish a method for determinating chlorogenic acid in Polygonum perfoliatum L.with high performance liquid chromatography（HPLC）.Methods： The HPLC method was carried out on C18（250 mm×4.6 mm,5 μm）column using acetonitrile-0.4% phosphoric acid（10∶90,v∶v） as mobile phase,and the flow rate was 1.0 ml · min^-1.Detection wavelength was 327 nm.Result： The calibration curve for chlorogenic acid was linear in the range of 0.042～1.14 μg.The average recovery was 99.19%（n=6）,and PSD was 1.7%.Conclusion： The method is convenient and accurate for quantitative determination of chlorogenic acid in P.perfoliatum L. |
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| Keywords: | Polygonum perfoltiarum chlorogenic acid chromatography high pressure liquid |
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