B7.1转基因肿瘤疫苗与大蒜素联合治疗膀胱癌的初步研究

目的初步估计大蒜素与B7.1转基因肿瘤疫苗对膀胱肿瘤的联合抗肿瘤效果.方法采用脂质体将B7.1基因导入鼠膀胱肿瘤(MBT-2)细胞.B7.1分子的表达经免疫荧光染色而测得.利用混合淋巴细胞培养MTT法,观察MBT-2-B7.1细胞刺激脾淋巴细胞的增殖作用.用MTT法测定了大蒜素对肿瘤细胞体外生长的影响.通过动物实验测定该肿瘤疫苗与大蒜素对膀胱肿瘤的联合治疗效果.结果转染了B7.1基因的肿瘤细胞能有效地刺激脾淋巴细胞增殖.当与大蒜素联合应用时,明显延迟了肿瘤的发生,并有效抑制了肿瘤的生长.结论大蒜素与转基因肿瘤疫苗治疗相结合能显著增强抗肿瘤效果.

Primary study of B7-1 modified tumor vaccine combined with Allicin for bladder cancer

Objective: This study primarily evaluated the anti-tumor effect of B7-1 modified tumor vaccine combined with Allicin for bladder tumor. Methods: A carcinogen-induced murine bladder cancer cell line, MBT-2, was transduced with lipofectin and B7-1 plasmid. The expression of B7-1 was confirmed by immunofluorescence staining. The ability of these modified cells was tested to stimulate in vitro the proliferation of syngeneic splenocytes from C3H/rHe mice. The direct cytotoxicity of Allicin in vitro was evaluated by MTT Assay. To determine whether B7-1modified tumor vaccine has a synthetic activity when it was combined with Allicin, subcutaneous bladder cancer were established in syngeneic C3H/He mice with 5×105 MBT-2 cells at the right flank. Treatments were started one week late. Mice were fed normal saline 0.1ml daily by gastric peffusion as a negative control. As in a treatment group, they were fed 2 mg Allicin daily, or injected parental MBT-2 cells or MBT-2-B7-1 cells subcutaneously at the opposite site as a vaccine, or injected MBT-2-B7-1 cells combined with Allicin gastric perfusion. Results: B7-1 modified tumor cells can stimulate splenocyte growth apparently. Combination Alliein-B7-1 gene therapy significantly inhibited the tumor growth compared with the controls, which was evident by decreased tumor incidence rate and delayed tumor growth. Conclusion: These results suggest that B7-1 modified tumor vaccine had a definite antitumor effect in a murine model of bladder cancer. Allicin treatment combined with modified tumor vaccine had significant additive anti-tumor effects. This research may provide a novel treatment modality for bladder cancer.