八氯二丙醚吸入染毒对小鼠脏器组织的氧化损伤作用

目的]研究八氯二丙醚(octachlorodipropyl ether)吸入染毒对小鼠肝、肾、脾、肺细胞的氧化损伤作用。方法]采用静式吸入染毒法,研究不同染毒羰基含量(282、564、1128 mg/m3,连续染毒20 d,每天1h)对小鼠的氧化损伤作用,染毒结束后测定各脏器丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及羰基含量。 结果] 高浓度(1128 mg/m3)染毒组中,小鼠肝、脾、肺MDA、羰基含量与对照组比差异有显著性(P<0．05);肺 MDA含量、羰基含量随染毒浓度的增加而增加,且呈剂量-反应关系(P<0．05)。高浓度组肝、脾、肺SOD、GSH-Px的活性下降且与对照组比差异有显著性(P<0．05);肾脏细胞各观察指标与对照组的差异均不显著。结论]八氯二丙醚吸入染毒可引起小鼠肝、脾、肺的氧化损伤,肺脏可能是其主要的靶器官。

Effect of Octachlorodipropyl Ether Inhalation on Oxidative Damage in Mice

Objective ] To study the effect of octachlorodipropyl ether(S2) inhalation on oxidative damage of liver, kidney, spleen and lung in mice. Methods ] Static inhalation chamber was applied for exposing mice to S2( 282, 564, 1128 mg/m3, 1h each day for 20 days ). Concentration of MDA, SOD, GSH-Px and carbonyl contents in some organs were detected to study oxidative damage of S2. Results ] In the high concentration group( 1128 mg/m3), MDA and carbonyl contents of liver, spleen and lung were significantly different from those in the control group( P < 0.05 ); MDA and carbonyl contents of lung increased with concentration of S2 (P<0.05 )with a dose-response relationship. In the high concentration group SOD and GSH-Px activities were reduced and significantly different to the control group (P<0.05 ); There were no significant difference in kidney between the test and control group (P>0.05 )for all of the oxidative damage indexes. Conclusion ] Inhalation of S2 can cause oxidative damage in liver, spleen and lung in mice, and lung might be the main target.