大剂量甲氨喋呤对小鼠骨髓基质细胞生物学特性的影响

目的：探讨大剂量甲氨喋呤（MTX）对小鼠骨髓基质细胞（BMSC）生物学特性的影响。方法：BALB／c小鼠按每千克的休重经尾静脉注射MTX0mg(对照组）、10mg（小剂量组）、40mg（大剂量组），化疗后取骨髓细胞进行分离培养，观察BMSC生长，成纤维细胞集落（CFU－F）（、BMSC层粘附率和造血细胞混合集落的变化。结果：小剂量组化疗后不同时间上述指标与对照组无明显差异，而大剂量组化疗后第7天开始出现BMSC层粘附率显著下降，第14天后出现生长减慢，CFU－F形成能力和造血支持功能降低，第35天仍低于正常，结论：大剂量化疗可导致BMSC损伤，且损伤持续时间较长。

Effects of high-dose methotrexate on biological properties of murine bone marrow stromal cells

Objective To explore the effects of high dose methotrexate (MTX) on murine bone marrow stromal cells (BMSC). Methods Each mouse was injected through caudal vein with MTX of 0 mg/kg (control group), 10 mg/kg (low dose group) or 40 mg/kg (high dose group). After chemotherapy bone marrow cells were harvested and cultured, then growth of BMSC, number of colony forming unit fibroblast (CFU F), adhesive rate of BMSC and growth of colony forming unit mixed hematopoietic cells on the BMSC layer were observed. Results The above mentioned indexes of low dose group were similar to those of the control group. In high dose group, however, the adhesive rate of BMSC began to decrease significantly on the 7th day and the growth of BMSC slowed down, the number of CFU F decreased and the ability to support hematopoiesis declined the 14th day. Until the 35th day the above mentioned indexes were lower than those of the control group. Conclusion BMSC can be damaged by high-dose chemotherapy and the effects can last longer.