MPTP诱导小鼠黑质神经元凋亡

目的　探讨 1-甲基 - 4苯基 - 1,2 ,3,6 -四氢吡啶 (MPTP)的神经毒性及其作用机制。　方法　 C5 7BL小鼠腹腔注射 MPTP,总量为 15 0 mg/kg,取中脑黑质 Nissl染色 ,TH免疫组织化学 ,TUNEL法和电镜观察黑质致密带的神经元改变。　结果　 MPTP处理后 ,黑质致密带的存活神经元和 TH阳性神经元的数目明显减少 (P<0 .0 1) ,TUNEL结果表明黑质神经元出现明显的 DNA断裂的特征性凋亡改变 (P<0 .0 1) ,黑质致密带神经元的超微结构出现早期凋亡改变。　结论　 C5 7BL小鼠腹腔注射 MPTP,可诱导黑质神经元凋亡 ,细胞凋亡是 MPTP的毒性作用方式之一。

MPTP Induce Apoptosis in Neurons of Mouse Substantia Nigra

Objective To study the neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP) and its mechanisms. Methods\ Twelve 6 to 8 weeks old C57BL male mice were administrated with MPTP(30 mg/kg,ip.) once every day for 5 days.\ Nissl staining, tyrosine hydroxylase(TH) immunohistochemistry staining, TUNEL tecnique and electronic microscopy were used to detect the neurotoxic effect of MPTP.\ Results\ Nissl staining and TH immunohistochemistry staining showed the significant loss of survived neurons and TH\|positive neurons in Pars compacta of substantia nigra(SNpc) after injection of MPTP(P<0 01).\ TUNEL staining showed apoptotic neurons with typical DNA fragmentation in SNpc(P<0 01).\ Electronic microscopy showed early phase changes of apoptosis in these neurons.\ Conclusion\ MPTP could induce apoptosis in the nigral neurons.\ Apoptosis was ascribed to the neurotoxicity of MPTP.