| HPLC-ELSD法测定芪灵扶正片中黄芪甲苷的含量 |
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| 引用本文： | 刘丹静,朱庄松,温海涛,陈繁华.HPLC-ELSD法测定芪灵扶正片中黄芪甲苷的含量[J].北方药学,2015(6):3-4. |
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| 作者姓名： | 刘丹静 朱庄松 温海涛 陈繁华 |
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| 作者单位： | 梅州市人民医院 梅州 514031;梅州市食品药品监督检验所 梅州514071 |
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| 摘 要： | 目的：探索建立高效液相色谱一蒸发光散射检测法（HPLC-ELSD）测定芪灵扶正片中的黄芪甲苷含量的方法。方法：采用色谱柱：依利特Hypersil ODS（5μm，4.6mm×200mm）；流动相：乙腈∶水（32∶68）；流速：1.0ml/min；ELSD参数：漂移管温度80℃；载气流量：2.0L/min。结果：黄芪甲苷在1.00～10.05μg的进样范围内线性关系良好，回归方程为y=1.3166x+21.2971（r=0.9999）；平均加样回收率为97.9%，RSD为1.8%。结论：该方法操作简单、快捷、准确，可作为芪灵扶正片的质量控制方法。
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| 关 键 词： | HPLC-ELSD 芪灵扶正片 黄芪甲苷 |
Determination of Astragaloside IV in Qiling Fuzheng Tablets by HPLC-ELSD |
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| Liu Danjing,Zhu Zhuangsong,Wen Haitao,Chen Fanhua.Determination of Astragaloside IV in Qiling Fuzheng Tablets by HPLC-ELSD[J].BEIFANG YAOXUE,2015(6):3-4. |
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| Authors: | Liu Danjing Zhu Zhuangsong Wen Haitao Chen Fanhua |
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| Abstract: | Objective To establish the determination of Astragaloside IV in Qiling Fuzheng Tablets by HPLC-ELSD. Methods This assay was performed on HypersilODS (5μm,4.6mm×200mm)column with acetonitrile-water (32∶68) as mobile phase in gradient elution at a flow rate of 1.0ml/min.The tube temperature of the detector was 80℃,and the flow rate was 2.0L/min. Results Astragaloside IV showed good linear correlation between 1.00 and 10.55μg (r=0.9999),with average recovery of 97.9%and RSD 1.8%. Conclusion The method is convenient, quick, accurate and sensitive, which can be used as controlmethod for Qiling Fuzheng Tablets. |
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| Keywords: | HPLC-ELSD Qiling Fuzheng Tablets Astragaloside IV |
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