Diagnosis of human rotavirus in stool specimens: comparison of different methods |
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Authors: | Paul S K Tabassum S Islam M N Ahmed M U Haq J U Shamsuzzaman A K |
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Affiliation: | Department of Microbiology, Mymensingh Medical College. |
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Abstract: | A total of 345 stool specimens of hospitalized children below 5 years of age with acute gastroenteritis were tested for the presence of rotavirus by Polyacrylamide gel electrophoresis (PAGE), a monoclonal antibody based enzyme immunoassay (EIA) and a latex agglutination test (LAT). Detection rate for PAGE and EIA were 24.9% (345/86) and 20.9% (345/70) respectively. Using PAGE as the standard, the sensitivity and specificity of EIA were 75.6% and 98.1% respectively. The sensitivity of LAT was 70.9% with 100% specificity (LAT was done in only PAGE positive specimens). LAT appeared as the simplest and economic for both bed side and field use. |
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