乙酰葛根素对大鼠局灶性脑缺血再灌注损伤的抗脂质过氧化作用

 目的探讨异黄酮类化合物乙酰葛根素对脑缺血再灌注损伤的保护作用及其机制。方法采用大脑中动脉阻塞法(MCAO)制备大鼠局灶性脑缺血再灌注模型。测定脑组织超氧化物歧化酶活性、丙二醛的含量和总抗氧化能力,并进行神经生物学评分。结果乙酰葛根素能使脑缺血大鼠的神经功能缺损评分明显低于缺血再灌组(P<0.01)。乙酰葛根素能明显降低缺血再灌注损伤后脑组织中的丙二醛含量,提高超氧化物歧化酶活性和总抗氧化能力。氯化三苯四氮唑染色显示,缺血再灌组在大脑皮质、基底节等均出现梗死灶。乙酰葛根素对缺血性脑梗死有改善作用。结论乙酰葛根素具有脑保护作用,其作用机制可能与抗氧自由基、抗脂质过氧化作用有关。

Protective Effects of Acetylpuerarin on Focal Brain Ischemia-Reperfusion Injury in Rats

OBJECTIVE To investigate the protective effect of acetylpuerarin(compound N-2211)on cerebral ischemia-reperfusion injury in rats and its mechanism.METHODS Brain ischemia/reperfusion injury model was prepared by MCAO method.The activity of superoxide dismutase(SOD),the level of malondialdehyde(MDA) and T-AOC were assayed with different methods respectively.RESULTS Compared with IR-group,the level of MDA and infarction size decreased,the activities of SOD and total antioxidative capacity(T-AOC)increased in acetylpuerarin treated rats.And the neurological deficit score was significant decreased in 1 h occlusion and 24 h middle cerebral artery reperfusion rats while sham operation rats had no residual deficit(P<0.01).Infarcted area was improved by acetylpuerarin in both cerebral cortex and basal ganglia in operation group stained by triphenyltetrazolium chloride staining method.CONCLUSION Acetylpuerarin possesses neural protection in brain ischemia/reperfusion damage.The positive effect of acetylpuerarin on brain focal ischemia injury may partly depend on its antioxidant property.