环氧化酶2在不同前列腺癌细胞系中的表达

目的:研究环氧化酶2(COX-2)在不同前列腺癌细胞系中的表达,探讨COX-2在前列腺癌侵袭进展及转移潜能获得机制中的可能作用。方法:应用Western印迹及RT-PCR鉴定LNCaP及其亚细胞系C4-2和AR-CaP亚细胞系IF11、IA8,以及PC-3细胞中COX-2的表达情况,并初步分析其在不同特性前列腺癌细胞系转移侵袭过程中的作用。结果:Western印迹结果显示:COX-2蛋白在PC-3细胞中表达相对较高,在IF11、IA8、LNCaP和C4-2细胞中表达缺失,差异具有统计学意义(P<0.05)。COX-2mRNA表达结果同蛋白一致。结论:不同来源、不同转移潜能的前列腺癌细胞株中COX-2表达存在差异。高表达COX-2可能在PC-3细胞高侵袭转移潜能获得方面起着一定作用,而与其他细胞系转移作用无关。

Expression of COX-2 in different human prostate cancer cell fines and its significance

Objective :To investigate the expression of cyclooxygenase-2 (COX-2) in different prostate cancer (PCa) cell lines and its role in the acquisition of invasive and metastatic potentials of PCa. Methods :We detected the expressions of COX-2 in prostate cancer cell lines LNCaP,C4-2,IF11,IA8 and PC-3 with different metastatic potentials by Western blotting and RT-PCR,and analyzed their roles in the invasion and metastasis of different PCa cell lines. Results :Western blotting and RT-PCR showed that the expression of the COX-2 protein was high in PC-3,but absent in IF11,IA8,LNCaP and C4-2 (P<0.05),and it was consistent with the expression of COX-2 mRNA. Conclusion :COX-2 expresses differently in PCa cell lines with different metastatic potentials. The overexpression of COX-2 may be associated with the high invasion and metastasis of PC-3,but not with the metastasis of other cell lines.