Initial adherence of human capillary endothelial cells to Dacron |
| |
| Authors: | J S Radomski B E Jarrell S K Williams E A Koolpe D A Greener R A Carabasi |
| |
| Affiliation: | 1. Miami University, USA;2. Michigan State University, NBER, and CES-Ifo, USA;3. Cornell University, NBER, and CES-Ifo, USA;1. Mechanical Engineering Department, King Fahd University of Petroleum & Minerals, Dhahran, Saudi Arabia;2. Center for Engineering Research, King Fahd University of Petroleum & Minerals, Dhahran, Saudi Arabia;3. Biology Department, King Fahd University of Petroleum & Minerals, Dhahran, Saudi Arabia;4. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States;1. The Center for Healthy Aging, Self-Management and Complex Care, College of Nursing, The Ohio State University, Columbus, OH, United States;2. Department of Health Behavior and Biological Sciences, School of Nursing, Michigan Society of Fellows 2022-2025, University of Michigan, Ann Arbor, MI,United States of America;3. Department of Systems, Populations and Leadership, School of Nursing, University of Michigan, United States of America |
| |
| Abstract: | Successful endothelialization of vascular grafts by seeding with endothelial cells (EC) at implantation is related to the number of EC which initially adhere to the graft. Using an in vitro system we examined the initial adherence of EC from human perinephric fat capillaries to woven Dacron that was either unmodified or precoated with several substrates. We studied capillary EC because they have not been investigated as a source of EC for graft seeding, although transinterstitial capillary ingrowth is one possible mechanism for spontaneous graft endothelialization. EC were isolated using collagenase and characterized morphologically and functionally including positive factor VIII-related antigen staining. EC were studied at three phases in culture: (A) primary EC with no subcultivations (EC-0); (B) EC after two subcultivations with trypsin (EC-2); and (C) EC after 10 subcultivations with trypsin (EC-10). EC were seeded onto graft material at a density of 10(5) cells/cm2 (100% confluence) and examined for cell counts and morphology after one day in culture by light and electron microscopy. Results are as follows: (table; see text) The conclusions are: (1) All capillary EC demonstrated adherence to Dacron, but this initial adherence was strongly influenced by graft pretreatment with collagen or plasma. (2) Serially subcultivated EC (EC-2 and EC-10) had significantly higher initial adherence to pretreated Dacron compared to the primary cells (EC-0) (P less than .05). This suggests that briefly cultured and subcultivated EC have superior initial adherence characteristics to treated dacron compared to primary EC with no subcultivations. (3) Fat capillary EC are easily procured and cultured and provide a rich source of human EC for endothelializing vascular prostheses. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
