TS、TP在非小细胞肺癌中的表达及临床意义

目的检测非小细胞肺癌标本中胸苷酸合成酶（Thymidylate Synthase,TS）,胸苷磷酸化酶（Thymidine Phosphorylase,TP）表达情况,评价TS、TP表达与患者年龄、性别、病理类型及肿瘤分期等的关系,探讨TS、TP的临床病理学意义。方法选择可手术的非小细胞肺癌患者,手术前未接受任何方案的化疗或放疗,采用免疫组织化学染色的方法对手术切取的非小细胞肺癌组织中TS、TP的表达进行检测。结果 85例标本中,分别有56.7%和70.8%的肿瘤组织TS、TP呈高表达,TS与TP表达呈明显正相关,TS表达水平与患者的年龄、性别、病理类型及肿瘤分期无关,TP表达水平与患者的年龄、性别、病理类型及肿瘤T分期,TNM总分期无关,但是,TP的表达水平在不同的N分期中存在显著差异。结论非小细胞肺癌组织中,TS、TP广泛过表达,TP表达水平与淋巴结转移相关,TP高表达可能促进淋巴结转移。

Significances of thymidylate synthase and thymidine phosphorylase expression in non-small cell lung cancer

Objective To evaluate the expression of thymidylate synthase（TS） and thymidine phosphorylase（TP） in non-small cell lung cancer（NSCLC） and its relationship with clinicopathological characteristics.Methods All of patients without anticancer treatment before surgery were confirmed by histopathology and the tumors were respectable.Immunohistochemistry was used to detect the TS and TP expression in tumor samples.Results Of the 85 tumor samples,47.2% and 82.7% was observed of TS and TP overexpression,respectively.TS expression had no correlation with age,sex,pathological subtypes and tumor stages.TP expression had no correlation with age,sex,pathological subtypes,T stages and TNM stages.TP expression was significantly correlated with the lymph node metastasis（P=0.015）.Conclusion TS and TP are highly expressed in NSCLC tissues.TP expression is related to N stage and may play a role in tumor metastasis.