参附注射液对大鼠肝缺血再灌注损伤时ATP酶的影响

目的:研究参附注射液对肝缺血再灌注损伤大鼠的保护作用及可能机制。方法:将36只大鼠随机分为参附注射液(SF)组与生理盐水对照(NS)组。肝脏缺血15min,分别于再灌注后1、3、24h取肝组织测定Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶水平,并行酶组织化学染色,观察Ca2+-ATP酶的活性变化。结果:再灌注1、3h后,SF组肝组织中Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶水平显著高于NS组;但在24h时,2组数据无显著性差异(P>0.05)。结论:参附注射液可以通过保护ATP酶发挥对大鼠肝缺血再灌注损伤早期保护作用。

Effects of Shen Fu Injection on Activity of ATPase in Hepatic Ischemia Reperfusion Injury in Rats

OBJECTIVE: To investigate the effects of Shen Fu Injection on the hepatic ischemia reperfusion injury in rats and explore its mechanism. METHODS: Thirty-six rats were randomly divided into Shen Fu Injection-treated group ( SF group) and physiological saline-treated group ( NS group) . After hepatic ischemia for 15 minutes and after reperfusion for 1 hour, 3 hours and 24 hours respectively, the hepatic tissue were resected for determining the activity of ATPase and for enzyme-histochemistry dyeing. RESULTS: After reperfusion for 1 hour and 3 hours, the levels of Na -K -ATPase and Ca2 -Mg2 -ATPase in the SF group became significantly higher than those in the NS group( P<0. 05) . But after 24 hours' reperfusion, there was no significant difference in the levels of Na -K -ATPase and Ca2 -Mg2 -ATPase in hepatic tissues between the SF group and the NS group( P>0. 05) . CONCLUSION: Shen Fu Injection can alleviate the hepatic ischemia repe_rfusion injury by protecting the activity of ATPase.