Inhibitory effect of extracts of Ginkgo biloba leaves on VEGF-induced hyperpermeability of bovine coronary endothelial cells in vitro |
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Authors: | Qiu Yan Rui Yao-Cheng Li Tie-Jun Zhang Li Yao Peng-Yuan |
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Affiliation: | DepartmentofPharmacology,SchoolofPharmacy,SecondMedicalMilitaryUniversity,Shanghai200433,China |
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Abstract: | AIM: To study whether extract of Ginkgo biloba (EGb) can protect against atherosclerosis. METHODS: Confluent monolayers of bovine coronary endothelial cells (BCECs), bovine coronary smooth muscle cells (BCSMCs), and cocultures of the two were incubated with medium containing VEGF and/or EGb, and flux of ^125I-labeled oxidized low density lipoprotein (ox-LDL) across the monolayers was measured. RESULTS: Incubation with VEGF significantly increased the permeability of BCEC monolayers to ^125I-ox-LDL in a time- and concentration-dependent manner, but had no effect on permeability of BCSMCs or endothelial cells-smooth muscle cells cocultures. EGb significantly inhibited the VEGF-induced hyperpermeability of BCECs. CONCLUSION: VEGF was important in the formation and development of atherosclerosis. The inhibition of VEGF-induced permeability by EGb suggests that extracts of Ginkgo biloba leaves may have important clinical applications in the treatment of cardiovascular diseases. |
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Keywords: | Ginkgo biloba vascular endothelium coronary vessals vascular endothelial growth factor capillary permeability iodine radioisotopes LDL lipoproteins |
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