银杏叶提取物对局灶性脑缺血再灌注后线粒体损伤的保护作用

目的研究杏花雨注射液对大鼠局灶性脑缺血再灌注后脑线粒体中SOD、ATPase、MDA的影响，探讨其抗脑缺血作用的机制。方法雄性SD大鼠63只，随机分为9组（n=7）。假手术组、4个缺血对照组（缺血2h分别再灌注0．5、1、2、4h组，其中缺血2h再灌注4h设为模型组），3个杏花雨注射液（A）治疗组（高、中、低剂量组），金纳多注射液（B）治疗对照组。治疗组分别于术后立即舌下静脉注射A5、10、20mg／kg，B10mg／kg。测定脑线粒体中SOD、ATPase活性和MDA含量、基质游离钙浓度。结果局灶性脑缺血再灌注后脑线粒体中MDA含量、基质游离钙浓度明显升高（P〈0．01），SOD、ATPase活性明显下降（P〈0．01）；A与B均能明显提高脑线粒体中SOD、ATPase活性（P〈0．01），减少MDA、游离钙（P〈0．01）；两种注射液同等剂量差异无显著意义（P〉0．05）。结论局灶性脑缺血再灌注后线粒体钙超载，自由基生成增加。杏花雨注射液可提高缺血再灌注大鼠脑线粒体总抗氧化活力，降低MDA、游离钙含量，保护缺血再灌注引起的神经元损伤。

The protective effect of Xinhani on mitochondria after focal brain ischemia-reperfusion in rats

Objective To study the effects of Xinhani solution on the concentraion of free Ca~(2+),SOD,ATPase,MDA in the brain mitochondria of rats during cerebral ischemia and reperfusion.Methods Sixty three sprague-dawley(SD) rats were divided randomly into nine groups:sham control group,injected intraperitoneally saline;Ischemia-reperfusion groups,including I(ischemia) 2h/R(reperfusion) 0.5,1,2,4h groups(I2h/P4h is vehicle group) saline;Ginaton(B) 10mg/kg treatment groups;Xinhani(A) 5,10,20mg/kg treatment groups.A,B were injected through sublingual vein of rats at the start of ischemia.The activities of SOD,ATPase and contents of MDA,concentration of free Ca~(2+) in the brain mitochondria of rats were detetmined.Results The brain mitochondria was injured remardably after postischemia reperfusion.Increasing of content of MDA,free Ca~(2+)(P<0.01),decreasing of the activity of SOD,ATPase(P<0.01) in mitochondria showed the injury of mitochondria.Compared vehicle to treatment groups,A and B injection could dose-dependently increase activities of SOD and ATPase(P<0.01) and decrease contents of MDA,free Ca~(2+)(P<0.01).There was no significant difference between A 10mg/kg groups and B 10mg/kg groups(P>0.05).Conclusion The results show that Xinhani injection solution could protect brain from ischemia-reperfusion injury with increase vitality to resist oxidation of brain tissue and decrease content of MDA.