哮喘儿童IgE T细胞亚群细胞因子的动态观察及临床意义

目的：探讨哮喘儿童血IgE和T细胞亚群、细胞因子的动态变化及临床意义。方法：应用免疫萤光法及双抗夹心酶联免疫吸附试验(ELISA)分析方法对45例哮喘儿童发作期和缓解期分别测定IgE,T细胞亚群和细胞因子。对照组为20例健康儿童。结果：哮喘发作期、缓解期CD_3~+,CD_4~+ T细胞及CD_4~+/CD_8~+高于对照组,差异有显著性(P<0.05或0.01),CD_8~+ T细胞与对照组比差异无显著性(P>0.05)。发作期CD_4~+ T细胞及CD_4~+/CD_8~+高于缓解期(P<0.05)。发作期IL-2,EFN-γ低于对照组(P<0.01或0.05),IL-4,IL-6,IL-8和IgE高于对照组(P<0.01或0.05);缓解期IL-2,IFN-γ低于对照组(P<0.01),IL-4,IL-8,IgE高于对照组(P<0.05或0.01),缓解期IL-6与对照组比较差异无显著性(P>0.05)。结论：儿童哮喘在发作期和缓解期均存在着免疫功能紊乱,提示儿童哮喘应长期抗变应性炎症治疗。 [中国当代儿科杂志,2003,5(1):23-26]

Serum Levels of IgE, T Cell Subgroups and Cytokines in Children with Bronchial Asthma

OBJECTIVE: To study the changes of serum levels of IgE, T-cell subgroups and cytokines in children with bronchial asthma during the stage of attack and convalescence. METHODS: The levels of T-cell subgroups were determined by the mono-clone antibody indirect-immuofluorence method and the levels of IgE, IL-2, IL-4, IL-6, IL-8 and IFN-γ were detected using ELISA in 45 children with asthma in the stage of attack and convalescence. Twenty healthy children served as the controls. RESULTS: Compared with the controls, there were higher levels of CD3+ and CD4+/ T cells and the ratio of CD4+/CD8+ in the 45 children with asthma in the stage of attack and convalescence ( P<0.05,P<0.01, P < 0.01, respectively), whereas there was no difference in the CD8+ T cell level. The CD4+ T cell level and the ratio of CD4+ /CD8+ of patients were higher in the stage of attack than those in the stage of convalescence ( P <0. 05). The levels of IL-2 and IFN-γ in the asthmatic children in the stage of attack were lower than those of the controls ( P <0.01 or 0.05) and the levels of IL-4, IL-6, IL-8 and IgE were higher than those of the controls ( P <0.01 or 0.05). In the stage of convalescence, the levels of IL-2 and IFN-γ were lower and levels of IL-4, IL-8 and IgE were higher than those of the controls ( P < 0.05 or 0.01), while no difference was noted in the IL-6 level. CONCLUSIONS: Children with bronchial asthma in the stage of both attack and convalescence have an imrnunologic imbalance. A longer-time anti-alergic treatment administrated for childhood asthma is necessary. [Chin J Contemp Pediatr, 2003, 5(1): 23-26]