| 日本血吸虫pcDNA3.1(-)/Sj20.8真核表达及其对小鼠保护性免疫作用 |
| |
| 引用本文： | 曾桥,肖建华,万志刚,廖力,张愉快,刘传爱,杨秋林.日本血吸虫pcDNA3.1(-)/Sj20.8真核表达及其对小鼠保护性免疫作用[J].中国寄生虫学与寄生虫病杂志,2006,24(2):153-155. |
| |
| 作者姓名： | 曾桥 肖建华 万志刚 廖力 张愉快 刘传爱 杨秋林 |
| |
| 作者单位： | 南华大学病原生物研究所,衡阳,421001 |
| |
| 摘 要： | 为检测日本血吸虫新基因Sj20.8作为核酸疫苗能否诱导小鼠产生保护性免疫作用， 用PCR法扩增Sj20.8基因序列， 构建重组质粒pcDNA3.1(-)/Sj20.8， 肌肉注射免疫小鼠后检测发现， Sj20.8能在小鼠肌肉中短时间存在并有微弱表达， 但未能诱导小鼠产生一定水平的保护性免疫。
|
| 关 键 词： | 日本血吸虫 cDNA文库 基因 Sj20.8 |
| 文章编号： | 1000-7423（2006）-02-0153-03 |
| 收稿时间： | 2004-08-10 |
| 修稿时间： | 2004年8月10日 |
Induction of Protective Immunity Against Schistosoma japonicumBy pcDNA3.1/Sj20.8 Nucleic Acid Vaccine in BALB/c Mice |
| |
| ZENG Qiao,XIAO Jian-hua,WAN Zhi-gang,LIAO Li,ZHANG Yu-kuai,LIU Chuan-ai,YANG Qiu-lin.Induction of Protective Immunity Against Schistosoma japonicumBy pcDNA3.1/Sj20.8 Nucleic Acid Vaccine in BALB/c Mice[J].Chinese Journal of Parasitology and Parasitic Diseases,2006,24(2):153-155. |
| |
| Authors: | ZENG Qiao XIAO Jian-hua WAN Zhi-gang LIAO Li ZHANG Yu-kuai LIU Chuan-ai YANG Qiu-lin |
| |
| Institution: | Institution of Pathogenic Biology, Nanhua University, Hengyang 421001, China |
| |
| Abstract: | Sj20.8 gene was amplified by PCR and inserted into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.1/Sj20.8,which was then injected into the quadriceps femoris of the BALB/c mice.Results showed that the Sj20.8 antigen was low expressed in the local tissue of the mice,and was not able to significantly reduce eggs in the liver than in the control mice. |
| |
| Keywords: | Sj20 8 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》浏览原始摘要信息 |
| 点击此处可从《中国寄生虫学与寄生虫病杂志》下载免费的PDF全文 |
