| 血管内皮生长因子对牛视网膜色素上皮细胞DNA合成的影响和榄香烯抗增殖作用的研究 |
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| 引用本文： | 接传红,郭彤,张惠蓉.血管内皮生长因子对牛视网膜色素上皮细胞DNA合成的影响和榄香烯抗增殖作用的研究[J].中国中医眼科杂志,2001,11(2):75-77. |
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| 作者姓名： | 接传红 郭彤 张惠蓉 |
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| 作者单位： | 1. 中国中医研究院眼科医院,
2. 北京大学第三医院眼科 |
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| 摘 要： | 目的探讨血管内皮生长因子(VEGF)对牛眼视网膜色素上皮(RPE)细胞DNA合成的影响，并观察榄香烯注射液的抗增殖作用。方法采用3H-TdR掺入法，在体外培养的牛眼RPE细胞中加入不同浓度的VEGF以及VEGF和榄香烯，应用液闪仪测定3H-TdR掺入的脉冲数(CPM)。结果不同浓度的VEGF均使CPM值明显增加，且与VEGF浓度呈剂量依赖关系，不同浓度的榄香烯均使RPE细胞的CPM值明显下降，且与榄香烯浓度呈剂量依赖关系。结论VEGF有促进RPE细胞的DNA合成的作用，榄香烯可抑制生长因子诱导的RPE细胞的DNA合成。
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| 关 键 词： | 视网膜色素上皮细胞 血管内皮生长因子 榄香烯 |
| 文章编号： | 1002-4379(2001)02-0075-03 |
| 修稿时间： | 2000年8月20日 |
A study on effect of vascular endothelial growth factor in DNA synthesis of retinal pigment epithelial cells and anti-proliferative effect of elemene |
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| JIE Chuanhong,GUO Tong,ZHANG Huirong.A study on effect of vascular endothelial growth factor in DNA synthesis of retinal pigment epithelial cells and anti-proliferative effect of elemene[J].Journal of Traditional Chinese Ophthalmology,2001,11(2):75-77. |
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| Authors: | JIE Chuanhong GUO Tong ZHANG Huirong |
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| Institution: | JIE Chuanhong\ 1,GUO Tong\ 2,ZHANG Huirong\ 21 Eye Hospital of China Academy of Traditional Chinese Medicine,Beijing 100040,China |
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| Abstract: | OBJECTIVE To investigate effect on VEGF in the cultured bovine RPE cells as well as the anti-proliferative effect of elemene METHODS VEGF or VEGF and elemene with different concentration were put in the cultured bovine RPE cells by 3H-TdR incorporation method, then the CPM value of 3H-TdR incorporation were determined according to liquid scintillation counter RESULTS VEGF of different concentration made the value of CPM increase significantly in a dose dependent manner Elemene of different concentration made the value of CPM of PRE cells decrease significantly in a dose dependent manner CONCLUSIONS VEGF promoted DNA synthesis of RPE cells and elemene inhibited the DNA synthesis of RPE cells which was induced by VEGF |
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| Keywords: | retinal pigment epithelial (RPE) cells vascular endothelial growth factor (VEGF) elemene |
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