神经生长因子对脑缺血再灌注后生长相关蛋白和突 …

目的 研究外源性神经生长因子（ＮＧＦ）对大鼠脑缺血再灌注后生长相关蛋白－４３）ＧＡＰ－４３）和突触素ｐ３８表达的影响。方法 成年健康雌性Ｗｉｓｔａｒ大鼠５４只，随机分为ＮＧＦ治疗组、对照组和假手术组。采用线栓法建立大脑中动脉缺血再灌注动物模型，应用免疫组织化学方法观察ＮＧＦ对脑缺血再灌注后ＧＡＰ－４３和ｐ３８表达的影响。结果 脑主射外缘性ＮＧＦ后，ＧＡＰ－４３和ｐ３８表达较对照组有所增高，但差异无

Effect of nerve growth factor on expression of growth associated protein and synaptophysin after cerebral ischemic reperfusion in rats

Objective To study the effects of exogenous nerve growth factor (NGF) on expressions of growth associated protein 43 (GAP 43) and synaptophysin p38 after middle cerebral artery occlusion/reperfusion in rats. Methods 54 adult female rats were divided randomly into NGF group, controlled group and sham operated group. The rats received left middle cerebral artery occlusion of 2 hours. The sections of the brains which were subjected 6 hours and 1,3,7,14,21days of reperfusion, and were processed by immunochemistry with antibodies against GAP 43 and p38. Results The expressions of GAP 43 and p38 were slightly increased after NGF treatment, but the differences were not remarkable(P> 0.05 ). Conclusion It is suggested that there might be neuroplasticity of regeneration and repairment in central nervous system after injury. The regulative effects of exogenous NGF on GAP 43 and p38 need further exploration.