Electrophysiological identification of principal and intercalated cells in the rabbit outer medullary collecting duct |
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Authors: | Bruce M. Koeppen |
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Affiliation: | (1) Division of Nephrology, Departments of Medicine and Physiology, University of Connecticut Health Center, 06032 Farmington, CT, USA |
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Abstract: | Intracellular microelectrode techniques were used together with inhibitors of Na+ transport (amiloride) and H+ transport (acetazolamide and SITS1) to identify principal cells and intercalated cells in the outer stripe of the rabbit outer medullary collecting duct. The principal cell (n=9) had a basolateral membrane voltage (Vbl) of –64.7±3.2 mV, a fractional resistance of the apical membrane (fRa=Ra/Ra+Rbl) of 0.82±0.02, and a K+-selective basolateral membrane. Luminal amiloride hyperpolarizedVbl by 10.3±2.1 mV and increasedfRa to near unity (n=7). Bath acetazolamide and SITS were without effect on these parameters. The intercalated cell (n=5) had aVbl of –25.0±3.2 mV, afRa of 0.99±0.01, and a Cl–-selective basolateral membrane. Bath acetazolamide or SITS hyperpolarizedVbl by 26.4±8.2 mV. Luminal amiloride did not alterVbl of this cell. The differential effects of the inhibitors also indicate that the principal and intercalated cells are probably not directly coupled electrically. |
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Keywords: | Collecting duct Intercalated cell Principal cell Electrophysiology Cell heterogeneity |
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