Immunohistochemical Heterogeneity Within Clinically Nonfunctioning Pituitary Adenomas |
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Authors: | Yamada Shozo Sano Toshiaki Takahashi Michie Shishiba Yoshimasa Teramoto Akira |
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Institution: | (1) Department of Endocrinology, Toranomon Hospital, Tokyo, Japan;(2) Department of Pathology, University of Tokushima School of Medicine, Tokushima, Japan;(3) Department of Neurosurgery, Toranomon Hospital, 2-2-2 Toranomon, Minato-ku, 105 Tokyo, Japan |
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Abstract: | To investigate whether adenohypophysial hormone expression is heterogeneous within individual clinically nonfunctioning pituitary
adenomas, immunohistochemical examinations were performed on tissues obtained by multiple sampling of 11 adenomas. Stained
sections were assessed by morphometric image analysis as well as semiquantitative estimation under microscopy. All tumors
except one were immunopositive for one or more gonadotropins. Results were divided into five grades based on the proportion
of immunoreactive cells per section. Semiquantitative estimation showed only a one-grade difference among samples from the
same tumor in four cases for FSHβ and in two cases for LHβ. These qualitative similarities between multiple samples were confirmed
by morphometric image analysis.
From the practical standpoint of making a diagnosis of nonfunctioning pituitary adenoma, it is not necessary to take into
account immunohistochemical heterogeneity within an individual tumor, and immunohistochemical findings in a given sample obtained
at surgery can be regarded as representative of the entire adenoma. |
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Keywords: | Immunohistochemistry nonfunctioning adenomas pituitary adenomas |
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