宫颈腺癌HPV16／18感染与ki67、p53蛋白表达的关系

目的研究人宫颈腺癌组织中ki67、p53蛋白表达，分析HPV16／18感染与ki67、p53蛋白表达的关系。方法采用组织微阵列技术结合原位杂交和免疫组化（二步法）检测24例慢性宫颈炎和86例宫颈腺癌HPV16／18-E6DNA和ki67、p53蛋白表达情况。结果HPV16／18-E6DNA与ki67、p53蛋白表达在宫颈腺癌组织中的阳性率分别为65．1％、51．2％、45．3％，均显著高于慢性宫颈炎组织8．3％、0．0％、0．0％（P〈0．01）。HPV16／18感染与宫颈腺癌的病理分级和组织学类型无关，但与ki67表达呈正相关（P〈0．05），与p53表达呈负相关（P〈0．05）。ki67、p53蛋白表达与宫颈腺癌的病理分级有关，G2、G3组阳性表达率均明显高于G1组（P〈0．05）。ki67、p53蛋白表达与宫颈腺癌组织学类型无相关性。结论宫颈腺癌的发生发展与HPV16／18感染及ki67、p53蛋白表达异常相关。

Relationship between the Infection of HPV16/18 and Expression of Ki67 and p53 Protein in Cervical Adenocarcinoma

Objective To study the expression of Ki67 and p53 proteins in cervical adenocarcinoma, and to analyze the relationship between the infection of human papillomavirus 16,18 （HPV16/18） and the expressions of ki67 and p53 proteins. Methods The expression of HPV16/18-E6DNA, Ki67 and p53 proteins were determined by tissue microarray combined with in situ hybridization and immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammation. Results The positive rates of HPV16/18-E6DNA,Ki67 and p53 proteins in cervical adenoearcinoma were 65.1% ,51.2% and 45.3% respectively,which were significantly higher than that in cervical chronic inflammation （8.3% ,0 and 0 ,P 〈 0.01 ）. HPV16/18 infection was positively correlated with Ki67 expression and negatively correlated with p53 expression. No correlation between the HPV16/18 infection and the pathological grades or histological subtypes existed in cervical adenoearcinoma. The expression of Ki67 and p53 proteins was correlated to the histological subtypes ,with significantly higher expression rate in G2 and G3 tumors than that in G1 tumors. Conclusion HPV16/18 infection and abnormal expression of ki67, p53 proteins might be involved in the carcinogenesis and development of cervical adenocarcinoma.