The effect of antioxidants on MNNG-induced stomach carcinogenesis in rats |
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Authors: | R. M. Balansky P. M. Blagoeva Z. I. Mircheva I. Stoitchev I. Chernozemskí |
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Affiliation: | (1) Laboratory of Chemical Carcinogenesis and Testing, Institute of Oncology, 1156 Sofia, Bulgaria |
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Abstract: | Summary The effect of vitamins A, C and E, butylated hydroxytoluene (BHT) and glutathione (GSH) on gastric carcinogenesis induced by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) was investigated. Male and female BD-VI rats 2–3 months old received a single oral application of MNNG dissolved in corn oil. The male rats were divided into four groups: Group-I: MNNG 250 mg/kg by intubation; Group-II: MNNG+ vitamin C daily in the drinking water (400 mg/l); Group-III: MNNG+vitamin C (400 mg/1) +100 g of milk broth (for each of 10 rats) containing vitamin A (40 000 IU), vitamin E (0.5 g) and BHT (0.1 g) three times a week. The treatment with antioxidants started 7 days before the MNNG administration and continued until the end of experiment. Group-IV rats received MNNG+oxyferriscorbone, i.p. as a single dose of 1.0 mg/kg, daily during the week before and the week after MNNG exposure and than 3 times a week till the end of the experiment. Female rats were divided into two groups: Group-I: MNNG 333 mg/kg by intubation; Group-II: MNNG +GSH orally at a dose of 100 mg/rat 1 h before and 5, 24, 48, and 72 h after MNNG intubation.The incidence of gastric tumors after 15 months of treatment was as follows: male rats, 82.4% in Group-I, 40.0% in Group-II, 40.7% in Group-III, and 50.0% in Group-IV; female rats; 72.7% in Group-I, and 36.0% in Group-II. |
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Keywords: | Single application of N-methyl-N-nitro-N-nitrosoguanidine, Antioxidants Inhibition of gastric carcinogenesis |
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