大剂量血脂康对兔血脂和动脉粥样硬化斑块的影响

目的探讨血脂康抗动脉粥样硬化的作用机制。方法健康日本大耳白兔48只于实验前适应性饲养2周，随机分为空白对照组8只，高脂模型组40只，空白对照组用普通兔饲料喂养10周；其余用高脂饲料（每1kg普通兔饲料加1％胆固醇和8％熟猪油）喂养10周。10周后从高脂模型组中随机抽取8只处死观察动脉斑块形成状况以确定造模是否成功。造模成功后高脂模型组32只随机分组，分为高脂对照组、血脂康普通剂量组、血脂康2倍剂量组、血脂康4倍剂量组各8只。各组饲料均改为普通饲料，用药前及12周后分别测血清总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、天冬氨酸氨基转移酶（AST）、肌酸激酶（CK）、低密度脂蛋白胆固醇（LDL-C），同时观察用药后主动脉组织病理形态学改变。结果血脂康各治疗组与高脂对照组相比，TC、TG、LDL-C降低、HDL-C升高，CK降低（均P〈0．01）；各组间AsT无统计学意义。病理形态观察，与空白对照组比较，高脂模型组主动脉壁有弥漫斑块和脂纹，血管内膜显著增厚，各用药组斑块面积明显减少，斑块／内膜面积比值与内膜／中膜厚度比值显著降低（P〈0．01）；且药物剂量越大效果越显著。结论普通剂量血脂康即可降低血清TC、TG、LDL-C水平，升高HDL-C，阻止动脉粥样硬化的进展，大剂量应用效果更佳。

Effects of high-dose xuezhikang on the progression of atherosclerosis and blood lipids in rabbits

Objective To evaluate the effect of Xuezhikang （XZK） in various doses on the progression of atherosclerosis and blood fat in rabbits and the possible mechanism- Methods Forty-eight Japanese white rabbits were included, fed on normal diets for two weeks. Then they were randomly divided into Normal Control Group （n = 8 ） and High Serum Lipid Group （n = 40）. The Normal Group stayed with normal diets for 10 weeks, and the High Serum Lipid Model Group was fed on high cholesterol diets （ normal diets ＋ 1% cholesterol ＋ 8% pig oil） for 10 weeks. After 10 weeks, the High Serum Lipid Model Group were randomly divided into five sub - groups： Model Group （ n = 8）, XZK Ordinary Dose Group （ n = 8 ）, XZK Double Dose Group （ n = 8） and XZK Quadruple Dose Group （ n = 8） . Each group was fed normal diets. Total cholesterol （TC）, Triglyceride （TG）, high density lipoprotein cholesterol （HDL-C）, aspartate aminotransferase （AST）, creatine kinase （CK）, low density lipoprotein cholesterol （ LDL-C ） in blood serum were measured before and after 12 weeks of treatment. The aortas were harvested for histomorphometry after 12 weeks of treatment. Results Compared with the Model Group, XZK decreased TC, TG, LDL-C, CK and increased HDL-C （ all P 〈 0. 01 ）. The area of atheroma decreased （P 〈 0. 01 ）. Histomorphometry showed the quantity of foam cells, atheromatous plaques area and intima-to-media in the Model Group were significantly increased compared with Normal Group （ P 〈 0. 01 ）. The quantity of foam cells, atheromatous plaques area and intima-to-media in XZK Ordinary Dose Group, XZK Double Dose Group and XZK Quadruple Dose Group were significantly decreased compared with the Model Group （ P 〈0. 01 ）. Meanwhile the effect of Large Dose Group was better than the Low Dose Group （P 〈 0. 05）. Conclusion XZK can prevent the development of atherosclerosis and decrease sennn lipid, and the effect of large dose XZK is better than low dose group.