L-精氨酸对糖尿病兔动脉损伤后内膜增生和内皮功能的影响

目的 :探讨糖尿病动脉损伤后内膜增生的机制和L 精氨酸对内膜增生及内皮功能的影响。方法 :2 8只糖尿病兔和 14只正常兔髂动脉行球囊损伤术后分为对照 (Con)组、糖尿病 (DM)组、L 精氨酸(L arg)组 ( 2 2 5 %L arg溶液饮水 )。术后 4周取髂动脉行图象分析或血管反应性测定 ,同时测定血浆中MDA、SOD和NO含量。结果 :DM组血浆中MDA水平明显低于Con组和L arg组 ,而SOD和NO水平明显降低。DM组内膜面积明显大于对照组 ( 0 2 5 5± 0 0 2 4vs.0 176± 0 0 2 9mm2 ,P <0 0 1) ,而L arg组内膜面积明显小于DM组 ( 0 15 3± 0 0 16,P <0 0 1)。L arg组髂动脉环对乙酰胆碱的舒张反应明显高于DM组。结论 :L arg能改善糖尿病兔的内皮功能 ,抑制动脉损伤后内膜增生

L-arginine inhibit intimal hyperplasia and ameliorate endothelial dysfunction after balloon injury in diabetic rabbit

Objective:To study the mechanisms of intimal hyperplasia after balloon injury in diabetic rabbit's iliac artery and obserre the effect of L\|arginine on endothelial dysfunction and intimal thickening.Method:28 diabetic rabbits and 14 normal rabbits were divided into control group,diabetic group and L\|arginine group (2 25% L\|arginine solution as their drinking water), and then their iliac arteries were injured by balloon.Four weeks after the injuries,the iliac arteries were got to do pathological analysis or vasoreactivity measurement,as well as the plasma MDA,SOD and NO levels measured.Result:The plasmal MDA levels in DM group were significantly higher than that of Con group and L\|arg group,as well as the plasma SOD and NO levels being significantly lower.The intimal areas of L\|arg group were significantly smaller than DM group(0 153±0 016 vs. 0 255±0 024 mm\+2, P <0 01),together with a better relaxation to acetylcholine in the injured iliac artery of L\|arg group than DM group (51 37±4 02% vs.34 08±3 64%, P <0 01).Conclusion:L\|arginine is active to ameliorate endothelial dysfunction and inhibit the intimal hyperplasia of the iliac artery after balloon injury in diabetic rabbit.