HPLC法测定芪归祛白胶囊中梣酮的含量

目的制定高效液相色谱法测定芪归祛白胶囊中梣酮含量的方法.方法色谱柱:ZorbaxC18柱(十八烷基硅烷键合硅胶为填充剂,4·6mm×250mm,5μm);柱温:室温(25℃);流动相:甲醇-水(56∶44);流速:1·0ml?min-1;进样量:20μl.检测波长:218nm.结果当梣酮浓度在1·5~9·0μg?ml-1范围内时呈良好的线性关系,回归方程:Y=3·4857×104X-1·3422×103,r=0·9997.平均回收率98·71%,RSD为1·09%,n=5.梣酮与其它杂质峰达基线分离,梣酮周围无杂质,分离度达2以上.结论采用高效液相色谱法测定芪归祛白胶囊中梣酮的含量,操作较简便、灵敏度高、阴性无干扰,能较好地控制芪归祛白胶囊中梣酮的含量.

Determination of fraxinellon in Qiguiqubai Capsule by HPLC

OBJECTIVE To establish a method for the determination of fraxinellon in Qiguiqubai Capsule.METHODS Zorbax C_ 18 cloumn(250mm×4.6mm,5μm)was used,column temperature:25℃,using methanol-water (56∶44)as a mobile phase(1.0ml·min~ -1 )and detection wavelength was at 218nm.RESULTS The calibration curve of fraxinellon was linear in the range of 1.5～9.0μg·ml~ -1 ,regression equation was Y=3.4857×104X-1.3422×10~3,r=0.9997 and the average recovery was 98.71% (RSD=1.06%,n=5).Fraxinellon line with other impurities peak reached essential separation of fraxinellon around without impurity separation reach 2 above.CONCLUSION The method was relatively simple,highly sensitive,accurate,no negative interference and can be used to control the quality of Qiguiqubai capsule.