针刺足阳明经穴对家兔胃窦平滑肌细胞舒缩长度的影响

目的 :从细胞水平研究足阳明经与胃相关的特异性。方法 :将 45只家兔随机分为生理盐水组、阿托品模型组、针刺足阳明经穴组、针刺足少阳经穴组、针刺足太阳经穴组 ,每组 9只。分组处理后 ,取胃窦平滑肌 ,制成活的单个平滑肌细胞悬液 ,在显微镜下以测微尺测定细胞舒缩长度。结果 :针刺足阳明经穴组的胃窦平滑肌细胞长度明显短于其他各组 (P <0 .0 1 )。结论 :针刺足阳明经穴能使经阿托品阻断的胃窦平滑肌细胞产生明显的收缩效应 ,进一步证实了足阳明经与胃之间存在特异性的联系。

Influence of Acupuncture of Acupoints of Foot-Yangming Meridian on the Diastolic Length of Gastric Antrum Smooth Myocytes in the Rabbit

Objective:To study the relatively-specific correlation be tween Foot-Yangming Mer idian and the stomach. Methods:45 rabbits anesthetized with ure thane were random ly divided into normal saline (NS) group, atropine model group, electroacupu ncture (EA) of Foot-Yangming Meridian “Zusanli”(ST 36), “Tianshu”(ST 25), “Li angmen”(ST 21) and “Sibai”(ST 2)] group, Foot-Shaoyang Meridian “Yang lingq uan”(GB 34), “Daimai”(GB 26), “Riyue”(GB 24) and “Yangbai”(GB 14)] group and Foot-Taiyang Meridian “Chengjin”(BL 56), “Cuanzhu”(BL 2), etc.] gro up, with 9 rabbits in each group. After EA treatment, the rabbit’s gastric antrum m uscle was taken to be incubated, and centrifuged for isolating smooth muscle cells, a nd then the cells were fixed with 2.5% glutaraldehyde and mounted to glass slice s. The cell length was detected under light microscope and the cellular contract ion percentage was calculated after treatment with atropine. Results:After treatment with atropine, the smooth myocyte length of atr opine model group (63.26±6.62 μm) was significantly larger than that of NS gro up ( 56.8 6±7.37 μm, P<0.05); while in comparison with model group, the cellular length of EA-Foot-Yangming group (39.16±2.33 μm) was obviously shorter (P<0 .01). No significant differences were found between model group and EA-Foot-S hao yang ( 59.63±8.31 μm), and between model group and EA-Foot-Taiyang group (58.41 ± 6.42 μm). Findings indicated that after administration of atropine, the g astr ic smooth myocytes were in relaxation state, after EA of acupoints of the Stomac h Meridian of Foot-Yangming, they were activated to induce contraction via sup pr essing M-receptor, suggesting that there exists a relatively specific correlat io n between Foot-Yangming Meridian and the stomach. Conclusion: Puncturing acupoin ts of Foot-Yangming Meridian can cause the smooth myocytes of rabbit gastric a ntrum to produce contraction.