刺五加皂苷对急性脊髓损伤后脊髓内BDNF和NGF表达的影响

［摘要］ 目的 研究刺五加皂苷对脊髓损伤后组织内脑源性神经营养因子（BDNF）和脊髓内神经生长因子（NGF）两种蛋白表达的影响，探讨刺五加皂苷对脊髓损伤后的保护作用。方法 将45只雄性大鼠随机分为3组：假手术组、模型组和刺五加皂苷干预组，每组15只。将实验动物用10%水合氯醛麻醉后运用改良Allen’s法进行急性脊髓损伤模型造模。造模后假手术组予正常饲养，模型组造模后进行连续7 d生理盐水腹腔注射，刺五加皂苷干预组造模后进行连续7天相应剂量的刺五加皂苷持续腹腔注射。ELISA法检测血清中丙二醛（MDA）和超氧化物歧化酶（SOD），通过病理切片分析大鼠脊髓组织病理变化，蛋白印迹法检测大鼠脊髓内BDNF和NGF表达。结果 与假手术组比较，模型组和刺五加皂苷干预组大鼠血清中MDA的含量增加（P﹤0.05），SOD的含量降低（P﹤0.05），脊髓有明显损伤，大鼠脊髓组织中的BDNF和NGF蛋白表达量上升（P﹤0.05）；与模型组相比，刺五加皂苷组大鼠后肢功能具有明显改善，血清中中MDA含量降低（P﹤0.05），SOD的含量升高（P﹤0.05），大鼠脊髓组织中的BDNF和NGF蛋白表达量上升（P﹤0.05）。结论 刺五加皂苷促进脊髓组织中神经元细胞内BDNF和NGF表达，利于损伤神经元的修复，对大鼠急性脊髓损伤具有保护作用。

Effect of acanthopanax senticosus saponins on expression of brain-derived neurotrophic factor and nerve growth factor in rat spinal cord after acute spinal cord injury

Objective To study the effect of acanthopanax senticosus saponins (ASS) on expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in rat spinal cord after spinal cord injury, so as to explore the protective effect of ASS against spinal cord injury. Methods A total of 45 male rats were evenly randomized into three groups: sham operation group, model group and ASS intervention group. The experimental animals were anesthetized with 10% chloral hydrate and then acute spinal cord injury model was made using modified Allen's method. After modeling the sham group received normal feeding, the model group was intraperitoneally given normal saline for 7 consecutive days, and the ASS intervention group was given appropriate dose of ASS for 7 consecutive days. Then ELISA was used to detect the serum malondialdehyde (MDA) and superoxide dismutase (SOD); the pathological changes of spinal cord tissue were observed, and the expression of BDNF and NGF was examined by Western blotting analysis. Results Compared with the sham group, the other two groups had significantly increased serum MDA (P<0.05) and significantly decreased SOD level (P<0.05), with notable spinal cord injury; besides, the two groups also had significantly increased expression of BDNF and NGF protein in the spinal cord tissue (P<0.05). Compared with the model group, ASS group had significantly improved spinal cord injury, with significantly decreased serum MDA content (P<0.05) and increased SOD content and BDNF, NGF expression in the spinal cord tissue (P<0.05). Conclusion ASS can promote BDNF and NGF protein expression in neurons of spinal cord, improving repair of damaged neurons and protecting spinal cord against acute injury in rats.