头花蓼不同极性部位抑菌作用的谱效学分析

目的:建立头花蓼醇提浸膏中7个有效部位的指纹图谱,阐明不同部位所有色谱峰代表的化学成分及其对抑菌效果的贡献值,揭示头花蓼抑菌的药效物质基础。方法:采用UPLC-TOF-MS建立头花蓼醇提浸膏中不同极性部位的指纹图谱,并对各色谱峰进行化学成分分析,通过96孔板法测得不同极性部位对大肠埃希菌、铜绿假单胞菌的最低抑菌浓度(MIC),利用偏最小二乘回归法(PLSR)建立不同极性部位指纹图谱与抑菌作用的谱效关系。结果:头花蓼不同极性部位对大肠埃希菌、铜绿假单胞菌抑菌效果成正相关的色谱峰分别为9个和13个,其主要抑菌成分为1~5和15,19号峰所代表的化合物。结论:头花蓼对大肠埃希菌、铜绿假单胞菌的主要抑菌活性部位为4个强极性部位(A,B,C,D),其主要抑菌成分为6-没食子酸酰葡萄糖,3,6-二没食子酸酰葡萄糖,1,3,6-三没食子酸酰葡萄糖和davidiin,为深入研究头花蓼的抑菌机制奠定了基础。

Spectrum-effect Relationship of Antibacterial Effect of Different Polar Part of Polygonum capitatum

Objective:To establish the fingerprints of 7 effective parts from Polygonum capitatum ethanol extract,and clarify the contribution of the chemical components represented by all peaks at different sites to the antibacterial effect,and then to reveal the material basis for the efficacy of P. capitatum. Method:UPLC-TOF-MS was introduced to analyze fingerprint of different polar part of P. capitatum ethanol extract,and the chemical constituents of all peaks were analyzed,96-well plate method was adopted to measure minimum inhibitory concentration(MIC) of different polar part for Escherichia coli and Pseudomonas aeruginosa.Partial least squares regression analysis was used to establish the spectrum-effect relationship. Result:Positive correlation peaks of different polar part for E. coli and P. aeruginosa were 9 and 13,respectively;its main antibacterial ingredients ranked as No. 1-5,15,19. Conclusion:The main antibacterial active sites of P. capitatum for E. coli and P. aeruginosa are four polar sites(A,B,C and D) with strong polarity,the main active ingredients are 6-galloylglucose,3,6-digalloylglucose,1,3,6-trigalloylglucose and davidiin.