石榴花的化学成分分离鉴定

目的:对石榴科石榴属植物石榴的干燥花瓣进行化学成分研究。方法:石榴花用60%乙醇提取,浓缩,依次用石油醚、二氯甲烷、乙酸乙酯、正丁醇进行萃取,对乙酸乙酯部位浸膏,通过硅胶柱、凝胶柱、半制备柱进行分离纯化,结合波谱数据和文献数据进行结构鉴定。结果:从石榴花乙酸乙酯部位浸膏中分离得到12个化合物,分别鉴定为没食子酸(1),没食子酸乙酯(2),木犀草素(3),8-甲雷杜辛(4),7-羟基-4',6,-二甲氧基异黄酮(5),刺芒柄花素(6),三粒小麦黄酮(7),蒲公英萜酮(8),齐墩果酸(9),乌苏酸(10),D-半乳糖醇(11),胡萝卜苷(12)。结论:其中化合物4~8,11为首次从石榴花(即维吾尔族药古丽娜)中分离得到,5,6,8是首次从该属植物中分离得到。

Chemical Constituents of Punica granatum Flower

Objective:To investigate the constituents of Punica granatum flower. Method:The P. granatum flowers were extracted with 60% ethyl alcohol and condensed to a small amount of volume. The extraction was carried out by petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol in turn. The compounds from ethyl acetate extract were isolated and purified by silica gel, semi-preparative HPLC, Sephadex LH-20 and recrystallization methods, while their structures were identified on the basis of spectral analysis and comparison of their spectral data with those in the literature. Result:Twelve compounds were isolated and identified respectively as gallic acid (1), ethyl gallate (2), luteolin (3), 8-O-methylretusin (4), 7-hydroxy-4'',6-dimethoxyisoflavone (5), formononetin (6), tricetin (7), taraxerone (8), oleanolic acid (9), ursolic acid (10), D-dulcitol (11), and daucosterol (12). Conclusion:The compounds 4-8 as well as 11 were isolated for the first time from P. granatum flower (ie. Uighur medicine Gulina),and compounds 5, 6 and 8 were isolated for the first time from this genus.