胃肠安对人胃癌裸鼠皮下移植瘤的抑制作用

目的:观察健脾中药胃肠安颗粒对人胃癌裸鼠皮下移植瘤的影响。方法:建立人胃癌裸鼠皮下移植瘤模型,随机分为生理盐水(生理盐水0.5 m L/只)组,胃肠安煎剂(生药35.49 g·kg-1)组,胃肠安颗粒(胃肠安颗粒3.54 g·kg-1)组,比较各组裸鼠瘤重情况,采用免疫组化法比较各组裸鼠瘤体Ki67抗原和增殖细胞核抗原(PCNA)蛋白表达,透射电镜检测各组瘤体细胞形态,脱氧核苷酸转移酶介导的末端标记法(TUNEL)检测各组瘤体细胞凋亡情况,酶联免疫吸附测定(ELISA)检测血清中肿瘤指标及酶学标志物。结果:胃肠安颗粒组和胃肠安煎剂组瘤体质量均低于空白组(P0.01);胃肠安颗粒组和胃肠安煎剂组PCNA,Ki67蛋白表达低于空白组(P0.01);透射电镜显示胃肠安颗粒组和胃肠安煎剂组皮下移植瘤细胞出现细胞凋亡、坏死并且凋亡指数均高于空白组,ELISA法显示胃肠安颗粒组与胃肠安煎剂组肿瘤标志物及酶学指标癌胚抗原(CEA),糖类抗原199(CA199),糖类抗原724(CA724),乳酸脱氢酶(LDH)和碱性磷酸酶(ALP)水平均低于空白组(P0.05)。结论:健脾中药胃肠安颗粒同胃肠安煎剂一样均可抑制人胃癌裸小鼠皮下移植瘤生长的作用,可能通过抑制肿瘤细胞增殖、诱导肿瘤细胞凋亡促进其分化而实现,其具体的机制需作进一步的研究。

Inhibitory Effect of Weichang'an Granule on Human Gastric Cancer Xenografts in Nude Mice

Objective:To study the effect of spleen tonifying traditional Chinese medicine(TCM) Weichang''an granule on human gastric cancer xenografts in nude mice. Method:The nude mice models of human gastric cancer were established in nude mice and randomly divided into normal saline group (0.5 mL/mouse), Weichang''an decoction group (35.49 g·kg^-1) and Weichang''an granule group (3.54 g·kg^-1). The tumor weight of nude mice between various groups was compared, and the expression levels of Ki67 and proliferating cell nuclear antigen (PCNA) in nude mice were compared by immunohistochemistry. The morphology of tumor cells was detected under transmission electron microscope, terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay was used to detect the apoptosis of tumor cells in each group, and serum tumor markers and enzymatic markers were detected by enzyme linked immunosorbent assay (ELISA). Result:The average tumor weight in the Weichang''an decoction group and the Weichang''an granule group were lower than those in normal saline group (P<0.01). The positive expression rates of PCNA and Ki67 were significantly lower than those in normal saline group (P<0.01), transmission electron microscopy showed that the apoptosis rate and the necrosis rate of the subcutaneous tumor cells were significantly higher than those in normal saline group, ELISA showed tumor markers and CEA, CA199, CA724 enzymes, lactate dehydrogenase gastrointestinal (LDH) and alkaline phosphatase (ALP) levels of the Weichang''an decoction group and the Weichang''an granule group were lower than those of the normal saline group (P<0.05). Conclusion:Weichang''an granule and Weichang''an decoction can inhibit the growth of gastric cancer implanted in nude mice by inhibiting tumor cell proliferation and inducing apoptosis of tumor cells and promoting their differentiation, the specific mechanism shall be further studied.