21种辛味中药挥发油透皮促渗效果的药性规律分析

目的:为初步探讨辛味中药挥发油透皮促渗的药性规律,选择21种有作为透皮吸收促进剂应用报道的常用辛味中药挥发油为研究对象,在同一研究平台上完成制备、理化性质考察和透皮促渗效果评价。方法:采用水蒸气蒸馏法制备19种中药挥发油,松节油和薄荷油为直接购置,测定其挥发性;采用GC-MS分析了挥发油的组成成分;利用改良的Franz扩散池法考察21种中药挥发油对模型药物布洛芬体外经皮透过性的影响;利用独立样本T检验和多重对应分析挥发性以及来源中药药性(四气、五味、归经)与透皮促渗效果的关系。结果:未发现挥发油的挥发性与透皮促渗效果之间存在相关性;四气中温、热两气对辛味中药挥发油透皮促渗效果的影响具有显著性差异,辛热中药挥发油的透皮促渗效果显著优于辛温中药挥发油(P0.05);具有辛苦味、归脾经的中药挥发油可能具有较强的透皮促渗效果。结论:辛味中药挥发油透皮促渗效果与来源中药的药性特征之间存在关联性,并以四气的影响最大。

Regulation of Transdermal Penetration Enhancement Effect of 21 Pungent Essential Oils Based on Drug Property Characteristics of Chinese Materia Medica

Objective:In this paper,21 kinds of pungent essential oils (EOs),which have been used as penetration enhancers(PE),were chosen to analyze the preliminary regulation between the penetration-enhancing effect of EOs and drug property characteristics of Chinese materia medica (CMM) on the same platform. Method:Firstly,19 EOs were extracted by steam distillation,while turpentine oil and peppermint oil were purchased and used directly.Then the volatility values of them were measured.The chemical compositions of EOs were analyzed by GC-MS.In vitro permeation studies with modified Franz diffusion cell method were applied to evaluate the transdermal penetration enhancement effect of the 21 EOs.Finally,independent sample T test,together with multiple correspondence analysis,were applied to analysis experimental data for the purpose to illustrate the relationship between volatility,drug properties of 21 EOs and the enhancing efficiency,respectively. Result:No correlation was found between the volatility and the permeation enhancing effect of EOs.However,four natures played an important role in promoting the transdermal absorption of ibuprofen,moreover,the penetration-enhancing effect of EOs with hot nature was significantly higher than that of warm nature (P<0.05).EOs with the properties of pungent and bitter,spleen channel might be superior in promoting activity towards the skin permeation of drugs. Conclusion:The penetration enhancing effect of pungent EOs are affected by drug property characteristics of CMM,especially four natures.