灰树花多糖对CIF模型磷酸化p38 MAPK表达的影响

目的:观察灰树花多糖对化疗相关性疲劳(CIF)模型疲劳程度的改善情况,并初步探讨其可能的机制。方法:6~8周龄SPF级C57BL/6小鼠60只随机分为4组,即空白组,模型组,灰树花多糖低剂量组(5 mg·kg~(-1))和灰树花多糖高剂量组(10 mg·kg~(-1)),每组15只。将除空白组以外的小鼠腹腔注射5-氟尿嘧啶(5-Fu,40 mg·kg~(-1)),每天给药1次,连续5 d,同时灰树花多糖低剂量组和灰树花多糖高剂量组给予不同剂量灰树花多糖灌胃,空白组和模型组给予等量生理盐水灌胃,期间测量体重和抓力变化。第15天处死动物取材测血尿素氮、血乳酸和肝糖原含量及腓肠肌p38丝裂原活化蛋白激酶(p38MAPK)mRNA和磷酸化p38 MAPK(p-p38 MAPK)的表达情况。结果:低剂量和高剂量的灰树花多糖均能明显增强CIF小鼠的抓力(P0.01);低剂量和高剂量灰树花多糖均可明显降低CIF小鼠运动后的血尿素氮值(P0.05);高剂量灰树花多糖可以明显降低CIF小鼠运动后的血乳酸值(P0.01);低剂量和高剂量灰树花多糖均可明显降低腓肠肌p38 MAPK mRNA和p-p38 MAPK蛋白的表达水平。结论:灰树花多糖可以有效改善化疗相关性疲劳,可能与下调p38 MAPK基因与p-p38 MAPK的表达有关。

Effect of Grifola Frondosa Polysaccharide on Expression of p-p38 MAPK in CIF Model

Objective:To investigate the effect of grifola frondosa polysaccharide in alleviating fatigue in the chemotherapy-induced fatigue (CIF) model, and explore its possible mechanism. Method:A total of 60 6-8 week-old SPF C57BL/6 mice were randomly divided into 4 groups, namely normal group, model group, low-dose grifola frondosa polysaccharide group (5 mg·kg^-1) and high-dose grifola frondosa polysaccharide group (10 mg·kg^-1). The mice were injected with 5-fluorouracil (40 mg·kg^-1), ip, once a day, for five days, except for normal group. At the same time, low-dose grifola frondosa polysaccharide group and high-dose grifola frondosa polysaccharide group were given different doses of grifola frondosa polysaccharide. Body weight and grasping power were measured during the experimental period. At the 15th day, the mice were put to death, and content of blood urea nitrogen, blood lactic acid and hepatic glycogen, as well as p38 mitogen-activated protein kinase (p38 MAPK) mRNA and protein expressions in gastrocnemius muscle were detected. Result:Both low-dose and high-dose grifola frondosa polysaccharide can obviously enhance the grasping power in CIF mice (P<0.01); low-dose and high-dose grifola frondosa polysaccharide can obviously decrease the value of blood urea nitrogen (P<0.05) after exercise. High-dose grifola frondosa polysaccharide can obviously decrease the blood lactic acid value in CIF mice after exercise (P<0.01), and low-dose and high-dose grifola frondosa polysaccharide can significantly reduce p38 MAPK mRNA and phosphorylated p38 MAPK protein expressions in gastrocnemius muscle. Conclusion:Grifola frondosa polysaccharide can improve chemotherapy-induced fatigue in mice, which may be correlated with down-regulation of p38 MAPK mRNA and phosphorylated p38 MAPK protein expressions.