基因重组酵母细胞测定中药中雌激素类化合物的研究

目的：研究不同性味中药中植物性雌激素的含量。方法：用重组人雌激素受体(hER)基因酵母细胞(W303-1A/hER-ERE-LacZ)测定清热药、解表药、利水渗湿药、止血药、补益药、收涩药中的植物性雌激素的活性。通过定量测定β-半乳糖苷酶活性表示中药的雌激素强度。结果：清热药中雌激素含量最高,为635×10^-3 nmol·g^-1,止血药与补益药中雌激素含量较低,几乎测不出。结论：虎杖、连翘、秦皮、黄芩、麦冬中可与雌激素受体结合的植物性雌激素含量较高。

Use W303-1A/hER-ERE-Lac Z to determine estrogenic compounds in traditional Chinese materia medica

OBJECTIVE: To study the content of phytoestrogen in dissimilarity herbs. METHOD: The activity of phytoestrogen in heat-clearing drugs, drugs for relieving exterior syndrome, diuretic, anastaltics, tonics and astringents were detected based on the recombinant yeast cell (W303-1A/hER-ERE-Lac Z). The estrogenic activity in traditional Chinese materia medica were assayed quantitatively by determining the expression of beta-galactosidase. RESULT: The phytoestrogen concentration (6.35 x 10(-3) nmol x g(-1) E2 equivalent) in heat-clearing drugs was the highest while that in anastaltic and tonic drugs was the lowest, which was less than the detected limit. CONCLUSION: Compared with the other traditional Chinese materia medica, the content of phytoestrogen, which can bind to estrogen receptor, in giant knotweed rhizome, forsythia suspense, ash bark, baical skullcap root and ophiopogonis tuber were higher.