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| 银杏内酯B对高糖诱导人晶状体上皮细胞凋亡的影响 | |
| 引用本文： | 王彦红,兰天野,陈婷,刘平,林丽丽. 银杏内酯B对高糖诱导人晶状体上皮细胞凋亡的影响[J]. 国际眼科杂志, 2016, 16(7): 1242-1244. DOI: 10.3980/j.issn.1672-5123.2016.7.09 |
| 作者姓名： | 王彦红 兰天野 陈婷 刘平 林丽丽 |
| 作者单位： | 哈尔滨医科大学附属第一医院眼科, 中国黑龙江省哈尔滨市,150001 |
| 基金项目： | 国家自然科学基金(No.30973275) |
| 摘 要： | 目的：探讨银杏内酯 B 对高糖诱导的人晶状体上皮细胞(human lens epithelial cells,HLECs)凋亡的影响。 方法：建立高糖诱导的 HLECs 模型,用不同浓度的银杏内酯 B 干预,MTT 比色法检测细胞存活率,Hochest33258染色观察凋亡细胞形态,透射电镜观察细胞内超微结构变化,比色法检测凋亡相关因子 Caspase-3及 Caspase-9的表达。 结果：高浓度葡萄糖可抑制 HLECs 活性,诱导 HLECs 发生凋亡,引起细胞内 Caspase-3及 Caspase-9表达水平升高；银杏内酯 B 能明显抑制高糖诱导的 HLECs 活力的下降,降低细胞凋亡率,减少高糖所致 HLECs 细胞内 Caspase-3及 Caspase-9的表达。 结论：银杏内酯 B 可能通过抑制 Caspase-3及 Caspase-9的表达而有效抑制了高糖诱导的 HLECs 凋亡。 |
| 关 键 词： | 银杏内酯 B 高糖 晶状体上皮细胞 凋亡 |
| 收稿时间： | 2016-03-09 |
| 修稿时间： | 2016-06-03 |
Effect of ginkgolide B on high glucose-induced human lens epithelial cell apoptosis |
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| Yan-Hong Wang,Tian-Ye Lan,Ting Chen,Ping Liu and Li-Li Lin. Effect of ginkgolide B on high glucose-induced human lens epithelial cell apoptosis[J]. International Eye Science, 2016, 16(7): 1242-1244. DOI: 10.3980/j.issn.1672-5123.2016.7.09 | |
| Authors: | Yan-Hong Wang Tian-Ye Lan Ting Chen Ping Liu Li-Li Lin |
| Affiliation: | Departmentof Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China,Departmentof Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China,Departmentof Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China,Departmentof Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China and Departmentof Ophthalmology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China |
| Abstract: | AIM: To study the effect of ginkgolide B on high glucose-induced human lens epithelial cell(HLEC)apoptosis. METHODS: The high glucose-induced HLEC model was established. Different concentrations of ginkgolide B were intervened. Cell viability was assayed by MTT assay, morphology of cell apoptosis was observed by hochest33258 staining. Cell ultrastructural changes were detected by transmission electron microscopy. Expressions of apoptosis-related factors caspses-3 and caspase-9 were tested by colorimetric detection. RESULTS: High glucose inhibited the activity of HLECs, induced apoptosis reaction of HLECs, caused high expression of apoptosis factors in cells; while ginkgolide B inhibited the decrease of cell viability induced by high glucose, decreased the HLEC apoptosis and reduced the expression of apoptosis factors Caspase-3 and Caspase-9. CONCLUSION: Ginkgolide B may inhibit the expression of caspses-3 and caspase-9 then effectively inhibited high glucose-induced apoptosis in HLECs. |
| Keywords: | ginkgolide B high glucose lens epithelial cells apoptosis |
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