| 高效液相色谱法测定香菊颗粒中黄芪甲苷含量 |
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| 引用本文： | 马雯霞,;衷红梅.高效液相色谱法测定香菊颗粒中黄芪甲苷含量[J].中国药业,2014(15):45-46. |
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| 作者姓名： | 马雯霞 ;衷红梅 |
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| 作者单位： | [1]西安医学院第二附属医院,陕西西安710038; [2]陕西省食品药品检验所,陕西西安710065 |
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| 摘 要： | 目的：建立测定香菊颗粒中黄芪甲苷含量的高效液相色谱法。方法色谱柱为 Kromasil C18柱（250 mm ×4.6 mm，5μm），流动相为乙腈-水（32：68），流速为1.0 mL / min，蒸发光散射检测器，气流为2.8 mL / min，漂移管温度为105℃，柱温为30℃。结果黄芪甲苷进样量在0.436~13.080μg 范围内与峰面积线性关系良好（ r =0.999），平均回收率为95.70%，RSD 为1.85%（ n =6）。结论该方法操作简便，精密度好，结果准确可靠。
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| 关 键 词： | 高效液相色谱法 黄芪甲苷 香菊颗粒 |
Determination of Astragaloside IV Content in Xiangju Granules by HPLC |
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| Institution: | Ma Wenxia, Zhong Hongmei (1. Second Affiliated Hospital, Xi＇ an Medical College, Xi＇ an, Shaanxi, China 710038; 2. Shaanxi Provincial Institute for Food and Drug Control, Xi＇ an, Shaanxi, China 710065) |
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| Abstract: | Objective To adopt the high performance liquid chromatography（HPLC） method to determine the astragaloside Ⅳ content in Xiangju Granules. Methods The chromatographic column was the Kromasil C18 column（250 mm × 4. 6 mm, 5 μm） with the mobile phase of acetonitrile - water（32 ： 68） at the column temperature of 30 ℃ . The flow rate was 1. 0 mL / min; ELSD; the drift tube temperature was set at 105 ℃ and the flow rate of gas was 2. 8 L / min. Results The linearity range of astragaloside Ⅳ was 0. 436 - 13. 080 μg（ r =0. 999 ）. The average recovery rate was 95. 70% （ n = 6）, RSD was 1. 85% （ n = 6） . Conclusion The method is easy to operate and has good precision with accurate and reliable detection results. |
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| Keywords: | high performance liquid chromatography （HPLC） sstragaloside Ⅳ Xiangju Granules |
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