液相色谱-串联质谱联用法测定心脑欣片中甜菜碱含量 |
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引用本文: | 赵勇,;王艳,;丁野,;郑金凤,;李文莉. 液相色谱-串联质谱联用法测定心脑欣片中甜菜碱含量[J]. 中国药业, 2014, 0(15): 39-41 |
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作者姓名: | 赵勇, 王艳, 丁野, 郑金凤, 李文莉 |
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作者单位: | [1]湖南省食品药品检验研究院,湖南长沙410001; [2]湖南中医药大学药学院,湖南长沙410208 |
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摘 要: | 目的:建立测定心脑欣片中甜菜碱含量的方法。方法采用液相色谱-串联质谱联用(LC - MS / MS)法测定甜菜碱的含量,以电喷雾离子源(ESI)正离子模式将样品离子化,多反应监测(MRM)模式下对甜菜碱( m / z 118.20→57.92和118.20→59.00)进行测定。色谱柱为 Venusil HILIC 柱(100 mm×2.1 mm,3μm),流动相为乙腈-水(80:20),流速为0.3 mL / min。结果甜菜碱进样质量浓度在4.44-177.6 ng / mL 范围内与峰面积积分值线性关系良好( r =0.9999),平均回收率为99.45%,RSD 为1.43%( n =6)。结论该方法简便、可靠、准确,可作为测定心脑欣片中甜菜碱含量的方法。
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关 键 词: | 心脑欣片 甜菜碱 液相色谱-串联质谱联用法 |
Determination of Betaine Content in Xinnaoxin Tablet by LC - MS/MS |
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Affiliation: | Zhao Yong, Wang Yan, Ding Ye, Zheng Jinfeng, Li Wenli(1. Hunan Provincial Institute for Food and Drug Control, Changsha, Hunan, China 410001; 2. Pharmacy College, Hunan University of Chinese Medicine, Changsha, Hunan, China 410208) |
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Abstract: | Objective To establish the method for determining the betaine contents in Xinnaoxin Tablet. Methods The betaine content in Xinnaoxin Tablet was determined by LC - MS / MS. The sample was ionized by the eletrospray ionization(ESI) positive ion mode and betaine( m / z 118. 20→57. 92 and 118. 20→59. 00) was detected under the multiple reaction monitor(MRM) mode. The chromatographic column was the Venusil HILIC column(100 mm × 2. 1 mm × 3 μm) . The mobile phase was acetonitrile - water(80 : 20), the flow rate was 0. 3 mL / min. Results The mass concentration of betaine sample size in the range of 4. 44 - 177. 6 ng / mL exhibited the good linear relation( r = 0. 999 9). The average recovery rate was 99. 45% , RSD 1. 43% ( n = 6). Conclusion This method is simple, reliable and ac-curate, which can be used to determine the betaine content in Xinnaoxin Tablet. |
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Keywords: | Xinnaoxin Tablet betaine LC - MS/MS. |
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