益阴消渴滴丸质量标准研究

目的建立益阴消渴滴丸的定性鉴别和含量测定方法。方法用薄层色谱（TLC）法定性鉴别益阴消渴滴丸中的葛根、荔枝核；用高效液相色谱（HPLC）法测定葛根中葛根素的含量，色谱条件为迪马C18柱（250mm×4．6mm，5μm），流动相为甲醇-水（25：75），检测波长为250nm柱温30℃。结果TLC色谱斑点清晰，分离度好，阴性对照无干扰；葛根素进样量在0．1738～1．7380μg范围内与峰面积积分值呈良好线性关系（r=0．9999），平均回收率为98．82％，RSD为0．37％。结论所建立的定性、定量分析方法可用于益阴消渴滴丸的质量控制。

Quality Standard of Yiyin Xiaoke Dropping Pills

Objective To establish the methods of qualitative identification and content determination in Yiyin Xiaoke Dropping Pills. Methods The TLC method was used to qualitatively identify Radix puerariae and Semen litchi; and the content of puerarin was determined by HPLC. The chromatographic parameters was the Diamonsil C18 column（250 mm×4.6mm,5μm）,methanol-water（25：75） as the mobile phase,the detection wavelength of 250 nm and the column temperature was 30 ℃. Results The developed TLC sports were clear with good separation and without negative interference;The linear range of puerarin were 0. 173 8- 1. 738 0μg（r=0.999 9）.The average recovery rate was 98.82%, RSD =0. 37%. Conclusion The established qualitative and quantitative analysis method is applicable for the quality control of Yiyin Xiauke Dropping Pills.