日本血吸虫未成熟卵对小鼠肝肉芽肿形成的免疫学影响

目的探讨日本血吸虫未成熟卵诱导宿主产生抗雌虫生殖和抗卵胚免疫的效果及其对小鼠肝肉芽肿形成的影响。方法采用未成熟虫卵可溶性抗原（ＳＩＥＡ）及其不同组分抗原免疫ＢＡＬＢ／ｃ小鼠，检测小鼠产生的抗病免疫能力。结果经ＳＩＥＡ和ＳＩＥＡ２６～２８０００分子抗原免疫后，均可明显地降低攻击感染后免疫鼠的肝肉芽肿数目和大小。与对照组比较，上述两者免疫后的小鼠，其肝肉芽肿平均直径分别下降了２９．２６％和４９．６４％，尤以ＳＩＥＡ２６～２８０００分子抗原的免疫效果为佳，且感染鼠脾重明显下降（Ｐ＜０．０１）。但是成熟虫卵可溶性抗原以及ＳＩＥＡ－１分部抗原免疫对降低肝卵肉芽肿数量和大小以及脾肿大程度均无明显作用，甚至刺激肉芽肿反应增强。结论ＳＩＥＡ和ＳＩＥＡ２６～２８０００分子抗原免疫可诱导宿主产生抗雌虫生殖和抗卵胚发育的作用，并明显抑制虫卵肉芽肿的形成。

Effects of induction of anti embryonation and anti fecundity immunity on liver granuloma formation in mice infected with schistosoma japonicum

Objective To investigate the effect of anti fecundity and anti embryonation immunity on liver granuloma formation in mice immunized with soluble immature egg antigen (SIEA) of schistosoma japonicum.Method BALB/c mice were immunized with the crude SIEA or 26 000 28 000 molecular antigen derived from SIEA and compared the size in schistosoma japonicum egg granulomas in the liver after challenge infection both the immunized mice and unimmunized controls.Results The number and size of egg granuloma were significantly decreased in mice immunized with SIEA and SIEA 26 28 000, by 29.26% and 49.64%, respectively. The inhibitive effects on granuloma formation and spleen weights in the group immunized with SIEA 26 28 000 were stronger than those in the group immunized with the crude SIEA. However, mean granuloma size and spleen weights in the group immunized with SEA or SIEA 1 fraction were not different from unimmunized control.Conclusion SIEA and SIEA 26 28 000 antigenic molecule play an important role in inhibition on liver granuloma formation through their anti embryonation and anti fecundity immunity effects.