单纯疱疹病毒Ⅱ型糖蛋白D基因Sav株及野生株的克隆和序列分析

探讨我国单纯疱疹病毒Ⅱ型（ＨＳＶ－２）糖蛋白Ｄ（ｇＤ）基因结构和功能及与国外的病毒株之间的差异，为研制我国生殖器疱疹疫苗提供理论依据。方法利用聚合酶链反应从ＨＳＶ－２Ｓａｖ国际标准毒株及我国１例生殖器疱疹复发型野生株中扩增、克隆出糖蛋白Ｄ基因序列。结果将野生株和Ｓａｖ株ＤＮＡ序列及氨基酸序列同ＨＳＶ－２Ｇ株比较，发现其ＤＮＡ同源性为９９．２％，氨基酸同源性为９９．１％。结论我国ＨＳＶ－２野生株同国外的Ｓａｖ株及Ｇ株ｇＤ基因存在高度的同源性，同时也存在一定变异。

Molecular Cloning and Sequence Analysis of Glycoprotein D Gene of Herpes Simplex Virus 2 Strain Sav and Wild Strain Isolated

Objective To study the diffrence of structure and function of HSV 2 gD gene among the wild strain, G and Sav strain. So as to provide theoretical basis for future production of HSV 2 vaccine. Method The partial glycoprotein D gene sequence of HSV 2 strain Sav and wild strain isolated from recurred genital herpes simplex were amplified and cloned with PCR. Results The homology comparision showed that the homology of DNA and amino acid were 99.2% and 99.1% respectively. Conclusions There are high homology of HSV 2 gD gene among the wild strain G and Sav strains,but some variations existed.