| 慢性应激致大鼠抑郁行为涉及海马形态变化和BDNF表达降低 |
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| 引用本文： | 王涵,李娜,文威,姜蓉,周岐新.慢性应激致大鼠抑郁行为涉及海马形态变化和BDNF表达降低[J].基础医学与临床,2011,31(5). |
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| 作者姓名： | 王涵 李娜 文威 姜蓉 周岐新 |
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| 作者单位： | 1. 重庆医科大学,药学院,药理教研室,重庆,400016
2. 重庆医科大学,基础医学院,干细胞与组织工程研究室,重庆,400016 |
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| 摘 要： | 目的 研究慢性不可预见轻度刺激(CUMS)致大鼠抑郁模型与海马病理形态改变和脑源性神经营养因子(BDNF)表达异常的关系.方法 大鼠分为对照组和模型组,应用CUMS建立大鼠抑郁模型;以开场(open-field)法、黄嘌呤氧化法和硫代巴比妥酸法、HE染色和免疫组化法分别检测大鼠行为、皮质MDA水平和SOD活力、海马病理形态和海马BDNF表达.结果 与对照大鼠相比,接受28 d CUMS大鼠开场实验得分明显降低,皮质SOD活力显著下降和MDA含量明显增高,海马神经元呈明显核固缩和BDNF表达显著降低(吸光度值803±422vs237±96,P<0.01).结论 CUMS致大鼠明显抑郁行为,其机制可能与神经元氧化-抗氧化系统失衡,自由基生成增加,由此致海马神经元受损和BDNF表达减少有关.
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| 关 键 词： | 抑郁症 慢性不可预见轻度刺激 海马 |
Depressive behavior of rats induced by CUMS involved in abnormal pathomorphology and BDNF expression in hippocampus of rats |
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| WANG Han,LI Na,WEN Wei,JIANG Rong,ZHOU Qi-xin.Depressive behavior of rats induced by CUMS involved in abnormal pathomorphology and BDNF expression in hippocampus of rats[J].Basic Medical Sciences and Clinics,2011,31(5). |
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| Authors: | WANG Han LI Na WEN Wei JIANG Rong ZHOU Qi-xin |
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| Institution: | WANG Han1,LI Na1,WEN Wei1,JIANG Rong2,ZHOU Qi-xin1(1.Dept of Pharmacology,Pharmacy School,2.Lab of Stem Cell and Tissue Engineering,Basic Medical School,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective Establish depression model of rat through chronically unpredicted mild stress(CUMS)in order to investigate its relationship with the change of hippocampal pathomorphology and Brain-derived neurotrophic factor(BDNF).Methods Rats were randomly divided into two groups,control and model.CUMS was used to develope the depression model of rats.The rat behavior,SOD activity and MDA level of cortex,hippocampal pathomorphology,and BDNF expression were tested by the methods of open-field,xanthine oxidase,TBA... |
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| Keywords: | BDNF |
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