| 双氢青蒿素人对急性髓系白血病HL-60细胞凋亡的诱导作用 |
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| 引用本文： | 陈伟,王玲,杜苑苑,曹东林,胡亮杉.双氢青蒿素人对急性髓系白血病HL-60细胞凋亡的诱导作用[J].广东医学,2011,32(13):1641-1643. |
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| 作者姓名： | 陈伟 王玲 杜苑苑 曹东林 胡亮杉 |
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| 作者单位： | 陈伟,王玲,杜苑苑,CHEN Wei,WANG Ling,DU Yuan-yuan(广东省第二人民医院,血液科,广州,510317);曹东林,CAO Dong-lin(广东省第二人民医院,检验科,广州,510317);胡亮杉,HU Liang-shan(南方医科大学珠江医院血液科,广州,510282) |
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| 基金项目： | 广东省自然科学基金资助项目 |
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| 摘 要： | 目的 探讨双氢青蒿素诱导人急性髓系白血病HL-60细胞凋亡的作用和机制.方法 用二甲氧唑黄细胞增殖及细胞毒性检测试剂盒检测双氢青蒿素对HL-60细胞生长的抑制作用,Annexin V-FITC/PI染色流式细胞仪检测细胞凋亡,流式细胞仪检测细胞内线粒体膜电位变化,分光光度法检测细胞Caspase-3活性变化.结果 双氢...
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| 关 键 词： | 双氢青蒿素 人急性髓系白血病 线粒体途径 凋亡 |
The induction dihydroartemisinin on apoptosis of human acute myeloblast leukemia HL-60 cells in vitro |
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| CHEN Wei,WANG Ling,DU Yuan-yuan,CAO Dong-lin,HU Liang-shan.The induction dihydroartemisinin on apoptosis of human acute myeloblast leukemia HL-60 cells in vitro[J].Guangdong Medical Journal,2011,32(13):1641-1643. |
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| Authors: | CHEN Wei WANG Ling DU Yuan-yuan CAO Dong-lin HU Liang-shan |
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| Abstract: | Objective To investigate the role of dihydroartemisinin in the apoptosis of human acute myeloblastic leukemia HL-60 cells. Methods The growth inhibition of HL-60 cells treated with dihydroartemisinin was assessed by XTT cell proliferation and cytotoxicity assay kit.The apoptosis and mitochondria membrane potential of HL-60 cells were assessed by flow cytometry with AnnexinV-FITC/PI and JC-1 staining,respectively.The activity of Caspase-3 was measured by ELISA. Results Dihydroartemisinin inhibited HL-60 cell... |
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| Keywords: | dihydroartemisinin human acute myeloblast leukemia mitochondria pathway apoptosis |
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