| 高效液相色谱测定皮肤消毒液中醋酸氯己定含量 |
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| 引用本文： | 卢素格,刘红丽,杨瑞春. 高效液相色谱测定皮肤消毒液中醋酸氯己定含量[J]. 中国消毒学杂志, 2011, 28(3) |
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| 作者姓名： | 卢素格 刘红丽 杨瑞春 |
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| 作者单位： | 河南省疾病预防控制中心,郑州,450016 |
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| 摘 要： | 目的建立皮肤消毒液中醋酸氯己定含量测定方法-高效液相色谱法。方法采用流动相稀释样品方式,用C8色谱柱进行皮肤消毒剂中醋酸氯己定含量色谱检测。结果采用C8色谱柱测定醋酸氯己定,有效改善了C18色谱柱测定时峰拖尾的弊端,提高了检测灵敏度。检测灵敏度为0.002~1.0 mg/m l浓度范围内,醋酸氯己定浓度与对应的峰面积呈现良好的线性关系(R=0.9999),样品加标回收率达96.0%~100.5%。结论该方法简单、快捷、灵敏度高,适用于消毒产品中醋酸氯己定的测定。
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| 关 键 词： | 醋酸氯己定 皮肤消毒剂 含量测定 高效液相色谱 |
DETERMINATION OF CHLORHEXIDINE ACETATE CONTENT IN SKIN DISINFECTANT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
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| LU Su-ge,LIU Hong-li,YANG Rui-chun. DETERMINATION OF CHLORHEXIDINE ACETATE CONTENT IN SKIN DISINFECTANT BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J]. Chinese Journal of Disinfection, 2011, 28(3) |
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| Authors: | LU Su-ge LIU Hong-li YANG Rui-chun |
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| Affiliation: | LU Su-ge,LIU Hong-li,YANG Rui-chun (Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016,China) |
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| Abstract: | Objective To establish a method to determination of chlorhexidine acetate content in skin disinfectant by HPLC.Method The sample was diluted by mobile phase and HPLC analyses were performed on a SunFire C8 column with 0.01mol/L KH2PO4 and acetonitrile as mobile phase.Results Chlorhexidine acetate in samples were well separated on C8 column by HPLC and the peak of chlorhexidine acetate on this column was sharp and symmetric.The results were satisfactory between 0.002mg/mL and 1.0mg/mL with a very good linear... |
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| Keywords: | chlorhexidine acetate skin disinfectant content determination high performance liquid chromatography |
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