| 穿心莲内酯对白念珠菌群感效应及相关毒力基因的影响 |
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| 引用本文： | 严园园,施高翔,邵菁,陆克乔,张梦翔,汪天明,王斌,汪长中. 穿心莲内酯对白念珠菌群感效应及相关毒力基因的影响[J]. 中国中药杂志, 2015, 40(2): 292-297 |
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| 作者姓名： | 严园园 施高翔 邵菁 陆克乔 张梦翔 汪天明 王斌 汪长中 |
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| 作者单位： | 安徽中医药大学 中西医结合临床学院, 安徽省中医药科学院 中西医结合研究所, 安徽 合肥 230038,安徽中医药大学 中西医结合临床学院, 安徽省中医药科学院 中西医结合研究所, 安徽 合肥 230038,安徽中医药大学 中西医结合临床学院, 安徽省中医药科学院 中西医结合研究所, 安徽 合肥 230038,安徽中医药大学 中西医结合临床学院, 安徽省中医药科学院 中西医结合研究所, 安徽 合肥 230038,安徽中医药大学 中西医结合临床学院, 安徽省中医药科学院 中西医结合研究所, 安徽 合肥 230038,安徽中医药大学 中西医结合临床学院, 安徽省中医药科学院 中西医结合研究所, 安徽 合肥 230038,安徽中医药大学 科研实验中心, 安徽 合肥 230038,安徽中医药大学 中西医结合临床学院, 安徽省中医药科学院 中西医结合研究所, 安徽 合肥 230038 |
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| 基金项目： | 国家自然科学基金项目(81073127);安徽中医药大学自然科研项目(2013zr009);安徽中医药大学引进人才项目(2013RC001) |
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| 摘 要： | 目的: 研究穿心莲内酯(andrographolide,AG)对白念珠菌群感效应(quorum sensing,QS)及相关毒力基因的影响. 方法: 气相色谱-质谱联用仪(GC-MS)检测AG干预白念珠菌不同时间后法尼醇和酪醇的含量变化;qRT-PCR检测QS调节的相关毒力基因CHK1,PBS2,HOG1的表达. 结果: 白念珠菌生长2 h时法尼醇和酪醇分泌量较少,AG作用后变化不明显;12 h后分泌量最高,24 h回落.不同浓度AG干预后,法尼醇含量降低,酪醇升高,呈现剂量依赖性,其中1 000 mg·L-1 AG效果均最明显.qRT-PCR结果显示,1 000 mg·L-1 AG在2,12,24 h时分别使CHK1下调2.375,3.330,4.043倍;PBS2下调2.010,4.210,4.760倍;HOG1变化不明显. 结论: AG可抑制法尼醇的分泌及促进酪醇的分泌,下调QS相关的毒力基因CHK1,PBS2的表达.
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| 关 键 词： | 穿心莲内酯 白念珠菌 群感效应 法尼醇 酪醇 |
| 收稿时间： | 2014-05-13 |
Effect of andrographolide on quorum sensing and relevant virulence genes of Candida albicans |
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| YAN Yuan-yuan,SHI Gao-xiang,SHAO Jing,LU Ke-qiao,ZHANG Meng-xiang,WANG Tian-ming,WANG Bin and WANG Chang-zhong. Effect of andrographolide on quorum sensing and relevant virulence genes of Candida albicans[J]. China Journal of Chinese Materia Medica, 2015, 40(2): 292-297 |
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| Authors: | YAN Yuan-yuan SHI Gao-xiang SHAO Jing LU Ke-qiao ZHANG Meng-xiang WANG Tian-ming WANG Bin WANG Chang-zhong |
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| Affiliation: | School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Integrated Traditional and Western Medicine Research Institution, Anhui Provincial Academy of Chinese Medical Sciences, Hefei 230038, China,School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Integrated Traditional and Western Medicine Research Institution, Anhui Provincial Academy of Chinese Medical Sciences, Hefei 230038, China,School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Integrated Traditional and Western Medicine Research Institution, Anhui Provincial Academy of Chinese Medical Sciences, Hefei 230038, China,School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Integrated Traditional and Western Medicine Research Institution, Anhui Provincial Academy of Chinese Medical Sciences, Hefei 230038, China,School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Integrated Traditional and Western Medicine Research Institution, Anhui Provincial Academy of Chinese Medical Sciences, Hefei 230038, China,School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Integrated Traditional and Western Medicine Research Institution, Anhui Provincial Academy of Chinese Medical Sciences, Hefei 230038, China,Science Experiment Center of Anhui University of Chinese Medicine, Hefei 230038, China and School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Integrated Traditional and Western Medicine Research Institution, Anhui Provincial Academy of Chinese Medical Sciences, Hefei 230038, China |
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| Abstract: | Objective: To investigate the effect of andrographolide (AG) on quroum sensing (QS) and relevant virulence genes of Candida albicans.Method: Gas-chromatography-mass spectrometry (GC-MS) was applied to detect the changes in the content of farnesol and tyrosol in C.albicans intervened by AG.The real-time quantitative PCR (qRT-PCR) was adopted to inspect the expressions of relevant virulence genes such as CHK1, PBS2 and HOG1 regulated by QS.Result: At 2 h after the growth of C.albican, the farnesol and tyrosol secretions reduced, without notable change after intervention with AG.The secretions were highest at 12 h and decreased at 24 h.After the intervention with different concentrations of AG, the farnesol content reduces, whereas tyrosol increased, indicating a dose-dependence, particularly with 1 000 mg·L-1AG.qRT-PCR revealed that 1 000 mg·L-1 AG could down-regulate CHK1 by 2.375, 3.330 and 4.043 times and PBS2 by 2.010, 4.210 and 4.760 times, with no significant change in HOG1.Conclusion: AG could inhibit the farnesol secretion, promote the tyrosol secretion and down-regulate QS-related virulence genes CHK1 and PBS2 expressions. |
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| Keywords: | andrographolide Candida albicans quroum sensing farnesol tyrosol |
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