| 4′-羰基乙氧苯基4-胍基苯甲酸酯盐酸盐对人精子顶体蛋白酶活性的影响 |
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| 引用本文： | 袁德祥,刁筱辉.4′-羰基乙氧苯基4-胍基苯甲酸酯盐酸盐对人精子顶体蛋白酶活性的影响[J].生殖医学杂志,1992(1). |
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| 作者姓名： | 袁德祥 刁筱辉 |
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| 作者单位： | 天津市计划生育研究所 邮政编码300191 |
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| 摘 要： | 应用明胶底物膜方法测定了4′-羰基乙氧苯基4-胍基苯甲酸酯盐酸盐(CEGB)对生育男子精子的明胶水解(顶体蛋白酶)活性的抑制作用。实验结果表明当底物膜中 CEGB 的浓度逐渐增加时,精子的晕圈形成率(SHF)逐渐减少,平均晕圈直径(MHD)和平均溶解面积(MLA)都逐渐缩小;当CEGB 的浓度增加到6μmol/L 时则可完全抑制精子的晕圈形成。结果表明 CEGB 能够抑制人精子的明胶水解活性。
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| 关 键 词： | 蛋白酶抑制剂 精子顶体蛋白酶 |
The inhibitory effect of 4′-carboethoxyphenyl 4-guanidinobenzoate hydrochloride on acrosin activity of human sperm |
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| Yuan Dexiang,Diao Xiaohui.Tianjin Municipal Research Institute for Family Planning.The inhibitory effect of 4′-carboethoxyphenyl 4-guanidinobenzoate hydrochloride on acrosin activity of human sperm[J].Journal of Reproductive Medicine,1992(1). |
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| Authors: | Yuan Dexiang Diao XiaohuiTianjin Municipal Research Institute for Family Planning |
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| Institution: | Yuan Dexiang,Diao Xiaohui.Tianjin Municipal Research Institute for Family Planning,300191 |
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| Abstract: | In vitro study was performed to evaluate the effect
of 4′-carboethoxyphenyl 4-guanidino benzoate hy-
drochloride(CEGB) on acrosin activity of human
sperm.A modified gelatin substrate film assay was
used to test the acrosin activity,marked by a halo for-
mation around the sperm head.A positive correlation
exists between the size of the halo,the lysis area(halo
formation) and the degree of acrosin activity.Infiltra-
tion of the gelatin substrate film with proteinase in-
hibitor CEGB in concertrations of 2,4,6,8,10/μmol/L
before adding sperm suspension showed reduction in
number of halo formations and in diameter of halo in
different degrees.Halo formation was completely sup-
pressed when CEGB was present at s concentration of
6μmol/L.The results indicated that a negative correla-
tion exists between the concentration of CEGB and the
acrosin activity(r=-0.995). |
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| Keywords: | Proteinase inhibitor Acrosin |
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