P27kip1与细胞周期素D3在非霍奇金淋巴瘤中的表达、定位及相互关系

目的探讨P27^kip1和细胞周期素（cyclin）D3在非霍奇金淋巴瘤（NHL）中的表达、定位、相互关系及意义.方法采用免疫组织化学方法检测100例NHL患者病理标本及20例反应性增生淋巴结标本中P27^kip1、cyclin D3及细胞增殖指标Ki-67的表达.采用Western blot、免疫荧光双标法和激光共聚焦技术检测3种淋巴瘤细胞系细胞内P27^kip1和cyclin D3的表达、定位.结果 P27^kip1在NHL组织中的阳性率低于反应性增生淋巴结,且与肿瘤侵袭性以及增殖活性（Ki-67指数,Ki-67 LI）呈负相关;cyclin D3在NHL组织中的阳性率高于反应性增生淋巴结,且与肿瘤侵袭性以及增殖活性呈正相关;P27^kip1与cyclin D3呈负相关.但在少数病例出现P27^kip1的异常高表达并伴有cyclin D3和Ki-67的高表达.P27^kip1与cyclin D3在3种淋巴瘤细胞系的表达水平不一,在Raji细胞中P27^kip1与cyclin D3共同高表达并且共定位.结论 P27^kip1的低表达,cyclin D3的高表达与多数NHL的发生发展有关;部分病例或细胞系中P27^kip1的异常高表达及其与cyclin D3相互作用可能是NHL发生的另一机制.

Expression, localization and interrelationship of P27kip1 and cyclin D3 in non-Hodgkin'''' s lymphoma

OBJECTIVE: To investigate the expression, localization and interrelationship of P27(kip1) and cyclin D3 in non-Hodgkin's lymphoma (NHL). METHODS: The expressions of P27(kip1), cyclin D3 and index Ki-67 was detected in 100 NHL and 20 reactive lymph nodes by immunohistochemical technique. The expression and localization of P27(kip1) and cyclin D3 in 3 NHL cell lines were detected by Western blot, double immunolabelling and laser scanning confocal microscopy, respectively. RESULTS: In general the expression of P27(kip1) in NHL was lower than in control group, and was negatively related to the tumor aggressiveness and proliferating activity; the expression of cyclin D3 in NHL was higher than in control group, and was positively related to the tumor aggressiveness and proliferating activity. There was a negative correlation between P27(kip1) and cyclin D3. Nevertheless, anomalous high P27(kip1) expression was found in a few NHL tissues with high expression of cyclin D3 and Ki-67. Overexpression and colocalization of P27(kip1) and cyclin D3 was found in Raji cell line. CONCLUSIONS: Under expression of P27(kip1) and overexpression of cyclin D3 may play a role in the occurrence and development of NHL. Anomalous high P27(kip1) expression and its interaction with cyclin D3 may be another mechanism for tumorogenesis of NHL.